This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pUC119-gRNA
catalog :
52255
citations: 2
Reference
Ly D, Iqbal S, Fosu Nyarko J, Milroy S, Jones M. Multiplex CRISPR-Cas9 Gene-Editing Can Deliver Potato Cultivars with Reduced Browning and Acrylamide. Plants (Basel). 2023;12: pubmed publisher
Li J, Norville J, Aach J, McCormack M, Zhang D, Bush J, et al. Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nat Biotechnol. 2013;31:688-91 pubmed publisher
product information
Catalog Number :
52255
Product Name :
pUC119-gRNA
article :
doi10.1038/nbt.2654
id8037
pubmed_id23929339
bacterial resistance :
Ampicillin
cloning :
backbonepUC119
backbone_mutation
backbone_origin
backbone_size3162
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
Other
Plant expression
growth notes :
gRNA target sequence GGACTTTTGCCAGCCATGGT This plasmid is used as a PCR template to assemble new desired guide RNA according to the strategy published in Figure S5 of our Nature Biotechnology paper.
growth strain :
Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.
origin :
37
pi :
alt_names
PDS3
phytoene desaturase 3
cloning
clone_methodRestriction Enzyme
cloning_site_3SmaI
cloning_site_5SacI
promoterArabidopsis U6 polymerase III promoter
sequencing_primer_35 ATTAAGTTGGGTAACGCC 3'
sequencing_primer_55 TGGAATTGTGAGCGGATA 3
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesAT4G14210, DL3145C, FCAALL.28, PDE226, PDS, PHYTOENE DESATURASE, PIGMENT DEFECTIVE 226, phytoene desaturase 3
genePDS3
id827061
genbank_ids
KF264452
mutation
nameguide RNA targeting AtPDS3
shRNA_sequence
size
species
3702
Arabidopsis thaliana
tags
resistance markers :
1884
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA