This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
HBT-pcoCas9
catalog :
52254
citations: 4
Reference
Westberg I, Carlsen F, Johansen I, Petersen B. Cytosine base editors optimized for genome editing in potato protoplasts. Front Genome Ed. 2023;5:1247702 pubmed publisher
Wu R, Lucke M, Jang Y, Zhu W, Symeonidi E, Wang C, et al. An efficient CRISPR vector toolbox for engineering large deletions in Arabidopsis thaliana. Plant Methods. 2018;14:65 pubmed publisher
Jansing J, Sack M, Augustine S, Fischer R, Bortesi L. CRISPR/Cas9-mediated knockout of six glycosyltransferase genes in Nicotiana benthamiana for the production of recombinant proteins lacking β-1,2-xylose and core α-1,3-fucose. Plant Biotechnol J. 2019;17:350-361 pubmed publisher
Li J, Norville J, Aach J, McCormack M, Zhang D, Bush J, et al. Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9. Nat Biotechnol. 2013;31:688-91 pubmed publisher
product information
Catalog Number :
52254
Product Name :
HBT-pcoCas9
article :
doi10.1038/nbt.2654
id8037
pubmed_id23929339
bacterial resistance :
Ampicillin
cloning :
backboneHBT-FLAG
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
Other
plant expression
growth strain :
transient expression of pcoCas9 gene in plant cells
origin :
37
pi :
alt_names
plant codon optimized Cas9
cloning
clone_methodRestriction Enzyme
cloning_site_3PstI
cloning_site_5BamHI
promoterhybrid constitutive promoter 35SPPDK
sequencing_primer_3sequencing from the terminator: 5 atcgcaagaccggcaacagga 3
sequencing_primer_5sequencing from the promoter: 5 gtcacgtagtaagcagctctcgg 3
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
KF264451
mutation
namepcoCas9
shRNA_sequence
size
species
100
Synthetic
tags
locationN terminal on insert
tagFLAG
locationN terminal on insert
tagNLS
locationC terminal on insert
tagNLS
resistance markers :
1884
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA