This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p(HA)HIF1alpha(401delta603) (L795V, C800S, L818S, L822V)
catalog :
52216
citations: 2
Reference
Li X, Wu Y, Liu A, Tang X. Long non-coding RNA UCA1 enhances tamoxifen resistance in breast cancer cells through a miR-18a-HIF1? feedback regulatory loop. Tumour Biol. 2016;37:14733-14743 pubmed
Koshiji M, Kageyama Y, Pete E, Horikawa I, Barrett J, Huang L. HIF-1alpha induces cell cycle arrest by functionally counteracting Myc. EMBO J. 2004;23:1949-56 pubmed
product information
Catalog Number :
52216
Product Name :
p(HA)HIF1alpha(401delta603) (L795V, C800S, L818S, L822V)
article :
doi10.1038/sj.emboj.7600196
id8031
pubmed_id15071503
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
mammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and LCLL mutation
origin :
37
pi :
alt_names
HIF1alpha (deltaODD)
hypoxia inducible factor 1, alpha subunit
HIF1alpha
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5KpnI
promoterCMV
sequencing_primer_3BGH Reverse
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
geneHIF1A
id3091
genbank_ids
mutationaa 401-603 deleted, removes the oxygen-dependent degradation (ODD) domain, and L795V, C800S, L818S, L822V mutations which that disrupt p300/CBP binding
nameHIF1alpha (401delta603) LCLL
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagHA
resistance markers :
1881
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA