p(HA)HIF1alpha(401delta603)R27G | Addgene 52215 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

p(HA)HIF1alpha(401delta603)R27G

catalog :

52215

citations: 2

Reference
Wu Y, Tang X, Lee S, Hong H, Cao X, Lau C, et al. Endothelial PPARδ facilitates the post-ischemic vascular repair through interaction with HIF1α. Theranostics. 2022;12:1855-1869 pubmed publisher
Koshiji M, Kageyama Y, Pete E, Horikawa I, Barrett J, Huang L. HIF-1alpha induces cell cycle arrest by functionally counteracting Myc. EMBO J. 2004;23:1949-56 pubmed

product information

Catalog Number :

52215

Product Name :

p(HA)HIF1alpha(401delta603)R27G

article :

doi	10.1038/sj.emboj.7600196
id	8031
pubmed_id	15071503

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3

backbone_mutation

backbone_origin

Invitrogen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

mammalian expression of HA tagged HIF1alpha with a deletion of the N-terminal activation domain and R27G mutation

origin :

pi :

alt_names

HIF1alpha (deltaODD)

hypoxia inducible factor 1, alpha subunit

HIF1alpha

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI
cloning_site_5	KpnI
promoter	CMV
sequencing_primer_3	BGH Reverse
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78
gene	HIF1A
id	3091

genbank_ids

mutation

aa 401-603 deleted, removes the oxygen-dependent degradation (ODD) domain, and R27G mutation which abolishes DNA binding

name

HIF1alpha (401delta603) R27G

shRNA_sequence

size

species

9606

Homo sapiens