This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
TGFB1-bio-His
catalog :
52185
citations: 4
Reference
Stachowski T, Snell M, Snell E. Structural insights into conformational switching in latency-associated peptide between transforming growth factor β-1 bound and unbound states. IUCrJ. 2020;7:238-252 pubmed publisher
Kiełbus M, Czapinski J, Kałafut J, Woś J, Stepulak A, Rivero Muller A. Genetically Engineered Lung Cancer Cells for Analyzing Epithelial-Mesenchymal Transition. Cells. 2019;8: pubmed publisher
Stachowski T, Grant T, Snell E. Structural consequences of transforming growth factor beta-1 activation from near-therapeutic X-ray doses. J Synchrotron Radiat. 2019;26:967-979 pubmed publisher
Sun Y, Vandenbriele C, Kauskot A, Verhamme P, Hoylaerts M, Wright G. A Human Platelet Receptor Protein Microarray Identifies the High Affinity Immunoglobulin E Receptor Subunit α (FcεR1α) as an Activating Platelet Endothelium Aggregation Receptor 1 (PEAR1) Ligand. Mol Cell Proteomics. 2015;14:1265-74 pubmed publisher
product information
Catalog Number :
52185
Product Name :
TGFB1-bio-His
article :
doi10.1074/mcp.M114.046946
id9831
pubmed_id25713122
bacterial resistance :
Ampicillin
cloning :
backbonepTT3
backbone_mutationRat CD4 d3+4, enzymatic biotinylation sequence, linker and His tag (CD4d3+4-BioLHis).
backbone_originYves Durocher, NRC
backbone_size6618
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Article ref 170
growth strain :
Expresses full-length Transforming growth factor beta-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
origin :
37
pi :
alt_names
Transforming growth factor beta-1 precursor
cloning
clone_methodRestriction Enzyme
cloning_site_3AscI
cloning_site_5NotI
promoterCMV
sequencing_primer_3
sequencing_primer_5CMV-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCED, DPD1, IBDIMDE, LAP, TGF-beta1, TGFB, TGFbeta
geneTGFB1
id7040
genbank_ids
mutation
nameTGFB1
shRNA_sequence
size2114
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagrat CD4 d3+4
locationC terminal on backbone
tagenzymatic biotinylation sequence
locationC terminal on backbone
tagHis tag
plasmid copy :
Insert was chemically synthesised.
resistance markers :
919
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA