murine TCR OTI-2A.pMIG II | Addgene 52111 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

murine TCR OTI-2A.pMIG II

catalog :

52111

citations: 5

Reference
Holling G, Chavel C, Sharda A, Lieberman M, James C, Lightman S, et al. CD8+ T cell metabolic flexibility elicited by CD28-ARS2 axis-driven alternative splicing of PKM supports antitumor immunity. Cell Mol Immunol. 2024;: pubmed publisher
Lucero O, Lee J, Bowman J, Johnson K, Sapparapu G, Thomas J, et al. Patient Specific Targeting of the T-cell Receptor Variable Region as a Therapeutic Strategy in Clonal T-Cell Diseases. Clin Cancer Res. 2023;: pubmed publisher
Miao T, Symonds A, Singh R, Symonds J, Ogbe A, Omodho B, et al. Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation. J Exp Med. 2017;214:1787-1808 pubmed publisher
Ghosh A, Smith M, James S, DAVILA M, Velardi E, Argyropoulos K, et al. Donor CD19 CAR T cells exert potent graft-versus-lymphoma activity with diminished graft-versus-host activity. Nat Med. 2017;23:242-249 pubmed publisher
Holst J, Szymczak Workman A, Vignali K, Burton A, Workman C, Vignali D. Generation of T-cell receptor retrogenic mice. Nat Protoc. 2006;1:406-17 pubmed

product information

Catalog Number :

52111

Product Name :

murine TCR OTI-2A.pMIG II

article :

doi	10.1038/nprot.2006.61
id	8000
pubmed_id	17406263

bacterial resistance :

Ampicillin

cloning :

backbone

pMIG II

backbone_mutation

backbone_origin

Dario Vignali, Addgene plasmid 52107

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

The cDNAs encoding the TCRa (lacking the stop codon) and TCRb chains were linked via a 2A peptide into a single open reading frame by recombinant PCR and cloned into a mouse stem cell virus (MSCV)-based retroviral vector containing an IRES-GFP cassette to facilitate the identification of marked cells. The 2A peptide remains attached to the TCRa carboxy terminus and can be used to verify the complete separation of the two chains. Inclusion of a Gly-Ser-Gly (GSG) spacer between TCRa and the 2A peptide ensures complete cleavage . We have not seen any deleterious effects of the 2A peptide on the function of any proteins examined thus far.

growth strain :

retroviral expression of both TCRalpha and TCRbeta using a single vector

origin :

pi :

alt_names

Valpha2

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	pLXSN5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Tcralpha
gene	Tcra
id	21473

genbank_ids

mutation

name

T cell receptor alpha chain

shRNA_sequence

size

species

10090

Mus musculus