This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCAG-hCas9D10A
catalog :
52101
citations: 1
Reference
Santo E, Paik J. A splice junction-targeted CRISPR approach (spJCRISPR) reveals human FOXO3B to be a protein-coding gene. Gene. 2018;673:95-101 pubmed publisher
product information
Catalog Number :
52101
Product Name :
pCAG-hCas9D10A
article :
doi
id8007
pubmed_id
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutationThe CMV promoter was replaced with CAG promoter.
backbone_originclontech
backbone_size5800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
The expression vector for humanized nickase Cas9 (Cas9D10A) under CAG promoter
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Not I
cloning_site_5Kpn I
promoterCAG
sequencing_primer_3TTGCATTCATTTTATGTTTCAGG
sequencing_primer_5CGGCTTCTGGCGTGTGACC
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationD10A
namehCas9D10A
shRNA_sequence
size4160
species
32644
Other
tags
locationC terminal on insert
tagSV40 NLS
plasmid copy :
hCas9D10A (Addgene: 41816), CAG promoter from Dr. Jun-ichi Miyazaki
resistance markers :
1823
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA