MSCV-shCasp2-IRES-GFP | Addgene 52061 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

MSCV-shCasp2-IRES-GFP

catalog :

52061

citations: 1

Reference
Oliver T, Meylan E, Chang G, Xue W, Burke J, Humpton T, et al. Caspase-2-mediated cleavage of Mdm2 creates a p53-induced positive feedback loop. Mol Cell. 2011;43:57-71 pubmed publisher

product information

Catalog Number :

52061

Product Name :

MSCV-shCasp2-IRES-GFP

article :

doi	10.1016/j.molcel.2011.06.012
id	7915
pubmed_id	21726810

bacterial resistance :

Ampicillin

cloning :

backbone

MSCV-IRES-GFP-Puro

backbone_mutation

backbone_origin

Addgene plasmid #20672

backbone_size

5900

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth strain :

stable knockdown of Caspase-2 in mammalian cells

origin :

pi :

GTATTAAAGAACAGGGAAGTTGGGATGCCTACTGCCTCGGAsizespecies

9606

Homo sapiens

tags

location	C terminal on backbone
tag	IRES-EGFP

shRNAshRNA_sequencetd>TGCTGTTGACAGTGAGCGCCCCAACTTCCCTG
TTCTTTAATAGTGAAGCCACAGAT

alt_names

caspase 2, apoptosis-related cysteine peptidase

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	EcoRI
promoter
sequencing_primer_3	pCDH-rev (GCATTCCTTTGGCGAGAG)
sequencing_primer_5	pLXSN5; pBABE5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CASP-2, ICH1, MRT80, NEDD-2, NEDD2, PPP1R57
gene	CASP2
id	835

genbank_ids

mutation

name

Caspase-2

resistance markers :

225

1871

tags :

Unknown

terms :

Puromycin