This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-myc3-Mdm2 p60
catalog :
52059
citations: 1
Reference
Oliver T, Meylan E, Chang G, Xue W, Burke J, Humpton T, et al. Caspase-2-mediated cleavage of Mdm2 creates a p53-induced positive feedback loop. Mol Cell. 2011;43:57-71 pubmed publisher
product information
Catalog Number :
52059
Product Name :
pCMV-myc3-Mdm2 p60
article :
doi10.1016/j.molcel.2011.06.012
id7915
pubmed_id21726810
bacterial resistance :
Ampicillin
cloning :
backbonepCMV
backbone_mutation
backbone_originClontech
backbone_size3800
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Original plasmid from Y. Xiong, Addgene plasmid #20935.
growth strain :
expression of truncated Mdm2 in mammalian cells
origin :
37
pi :
alt_names
MDM2 oncogene, E3 ubiquitin protein ligase
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5BamHI
promoterCMV
sequencing_primer_3
sequencing_primer_5Bglob-intron-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesACTFS, HDMX, LSKB, hdm2
geneMDM2
id4193
genbank_ids
mutationp60 (truncated at D367, DVDP motif)
nameMdm2 p60 (truncated)
shRNA_sequence
size1100
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagmyc3
resistance markers :
225
1871
tags :
Unknown
terms :
Neomycin (select with G418)
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA