This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDYH5
catalog :
52032
citations: 1
Reference
Tao L, Xie Q, Ding Y, Li S, Peng S, Zhang Y, et al. CAMKII and calcineurin regulate the lifespan of Caenorhabditis elegans through the FOXO transcription factor DAF-16. elife. 2013;2:e00518 pubmed publisher
product information
Catalog Number :
52032
Product Name :
pDYH5
article :
doi10.7554/eLife.00518
id7988
pubmed_id23805378
bacterial resistance :
Ampicillin
cloning :
backbonepPD95.81
backbone_mutation
backbone_originAndrew Fire (Addgene Plasmid 1497)
backbone_size4406
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
growth notes :
The D->V point mutation in DAF-16 has no functional consequence.
growth strain :
Expression of GFP-tagged DAF-16 in C. elegans
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5NotI
promoter
sequencing_primer_3GFP-R
sequencing_primer_5M13-Rev
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_R13H8.1
genedaf-16
id172981
genbank_ids
mutation
nameDAF-16
shRNA_sequence
size7672
species
6239
Caenorhabditis elegans
tags
locationC terminal on insert
tagHIS
locationC terminal on insert
tagGFP
plasmid copy :
Insert DNA a gift from Thomas E. Johnson (University of Colorado, Boulder)
resistance markers :
1863
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA