This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCS2+hSpCas9
catalog :
51815
citations: 28
Reference
Kiyomitsu A, Nishimura T, Hwang S, Ansai S, Kanemaki M, Tanaka M, et al. Ran-GTP assembles a specialized spindle structure for accurate chromosome segregation in medaka early embryos. Nat Commun. 2024;15:981 pubmed publisher
Altb xfc rger C, Holzhauser J, Driever W. CRISPR/Cas9-based QF2 knock-in at the tyrosine hydroxylase (th) locus reveals novel th-expressing neuron populations in the zebrafish mid- and hindbrain. Front Neuroanat. 2023;17:1196868 pubmed publisher
Fu B, Wu D, Yasumasu S, Hane M, Sato C, Kitajima K. Critical Role of the Cortical Alveolus Protease Alveolin in Chorion Hardening In Vivo at Medaka Fertilization. Biomolecules. 2023;13: pubmed publisher
Fleming T, Kikuchi Y, Nakajo M, Tachizawa M, Inazumi T, Tsuchiya S, et al. Prostaglandin E2 receptor Ptger4b regulates female-specific peptidergic neurons and female sexual receptivity in medaka. Commun Biol. 2022;5:1215 pubmed publisher
Shirai M, Takayama K, Takahashi H, Hirose Y, Fujii M, Awazu A, et al. Methylome data derived from maternal-zygotic DNA methyltransferase 3aa-/- zebrafish. Data Brief. 2022;44:108514 pubmed publisher
Ertunc N, Phitak T, Wu D, Fujita H, Hane M, Sato C, et al. Sulfation of sialic acid is ubiquitous and essential for vertebrate development. Sci Rep. 2022;12:12496 pubmed publisher
Ogawa Y, Shiraki T, Fukada Y, Kojima D. Foxq2 determines blue cone identity in zebrafish. Sci Adv. 2021;7:eabi9784 pubmed publisher
Nakanishi E, Uemura N, Akiyama H, Kinoshita M, Masanori S, Taruno Y, et al. Impact of Gba2 on neuronopathic Gaucher's disease and α-synuclein accumulation in medaka (Oryzias latipes). Mol Brain. 2021;14:80 pubmed publisher
Kato Unoki Y, Takai Y, Nagano Y, Matsunaga S, Enoki S, Takamura T, et al. Production of a tributyltin-binding protein 2 knockout mutant strain of Japanese medaka, Oryzias latipes. Mar Pollut Bull. 2020;160:111601 pubmed publisher
Kikuchi M, Nishimura T, Ishishita S, Matsuda Y, Tanaka M. foxl3, a sexual switch in germ cells, initiates two independent molecular pathways for commitment to oogenesis in medaka. Proc Natl Acad Sci U S A. 2020;117:12174-12181 pubmed publisher
Watanabe Y, Okuya K, Takada Y, Kinoshita M, Yokoi S, Chisada S, et al. Gene disruption of medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2) causes movement retardation. J Biochem. 2020;: pubmed publisher
Lee Y, Kawakami K, HuangFu W, Liu I. Chondroitin sulfate proteoglycan 4 regulates zebrafish body axis organization via Wnt/planar cell polarity pathway. PLoS ONE. 2020;15:e0230943 pubmed publisher
Asakawa K, Handa H, Kawakami K. Optogenetic modulation of TDP-43 oligomerization accelerates ALS-related pathologies in the spinal motor neurons. Nat Commun. 2020;11:1004 pubmed publisher
Shibata Y, Bao L, Fu L, Shi B, Shi Y. Functional Studies of Transcriptional Cofactors via Microinjection-Mediated Gene Editing in Xenopus. Methods Mol Biol. 2019;1874:507-524 pubmed publisher
Shiraki T, Kawakami K. A tRNA-based multiplex sgRNA expression system in zebrafish and its application to generation of transgenic albino fish. Sci Rep. 2018;8:13366 pubmed publisher
Kato Unoki Y, Takai Y, Kinoshita M, Mochizuki T, Tatsuno R, Shimasaki Y, et al. Genome editing of pufferfish saxitoxin- and tetrodotoxin-binding protein type 2 in Takifugu rubripes. Toxicon. 2018;153:58-61 pubmed publisher
Asakawa K, Kawakami K. Protocadherin-Mediated Cell Repulsion Controls the Central Topography and Efferent Projections of the Abducens Nucleus. Cell Rep. 2018;24:1562-1572 pubmed publisher
Nagao Y, Takada H, Miyadai M, Adachi T, Seki R, Kamei Y, et al. Distinct interactions of Sox5 and Sox10 in fate specification of pigment cells in medaka and zebrafish. PLoS Genet. 2018;14:e1007260 pubmed publisher
Watanabe Y, Furukawa E, Tatsukawa H, Hashimoto H, Kamei Y, Taniguchi Y, et al. Higher susceptibility to osmolality of the medaka (Oryzias latipes) mutants in orthologue genes of mammalian skin transglutaminases. Biosci Biotechnol Biochem. 2018;82:1165-1168 pubmed publisher
Yeh Y, Kinoshita M, Ng T, Chang Y, Maekawa S, Chiang Y, et al. Using CRISPR/Cas9-mediated gene editing to further explore growth and trade-off effects in myostatin-mutated F4 medaka (Oryzias latipes). Sci Rep. 2017;7:11435 pubmed publisher
Murakami Y, Ansai S, Yonemura A, Kinoshita M. An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes). Zoological Lett. 2017;3:10 pubmed publisher
Horimizu R, Ogawa R, Watanabe Y, Tatsukawa H, Kinoshita M, Hashimoto H, et al. Biochemical characterization of a medaka (Oryzias latipes) orthologue for mammalian Factor XIII and establishment of a gene-edited mutant. FEBS J. 2017;284:2843-2855 pubmed publisher
Ansai S, Kinoshita M. Genome Editing of Medaka. Methods Mol Biol. 2017;1630:175-188 pubmed publisher
Kawahara A. CRISPR/Cas9-Mediated Targeted Knockin of Exogenous Reporter Genes in Zebrafish. Methods Mol Biol. 2017;1630:165-173 pubmed publisher
Ota S, Kawahara A. Detection of Multiple Genome Modifications Induced by the CRISPR/Cas9 System. Methods Mol Biol. 2016;1451:53-63 pubmed publisher
Shah A, Moens C, Miller A. Targeted candidate gene screens using CRISPR/Cas9 technology. Methods Cell Biol. 2016;135:89-106 pubmed publisher
Tokumasu Y, Iida A, Wang Z, Ansai S, Kinoshita M, Sehara Fujisawa A. ADAM12-deficient zebrafish exhibit retardation in body growth at the juvenile stage without developmental defects. Dev Growth Differ. 2016;58:409-21 pubmed publisher
Ansai S, Kinoshita M. Targeted mutagenesis using CRISPR/Cas system in medaka. Biol Open. 2014;3:362-71 pubmed publisher
product information
Catalog Number :
51815
Product Name :
pCS2+hSpCas9
article :
doi10.1242/bio.20148177
id7957
pubmed_id24728957
bacterial resistance :
Ampicillin
cloning :
backbonepCS2
backbone_mutationCloned in hSpCas9 expression sequence
backbone_origin
backbone_size4071
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
Other
medaka, zebrafish
growth notes :
This plasmid is modified from pX330-U6-Chimeric BB-CBh-hSpCas9 originated from Feng Zhang Lab.
growth strain :
Encode hSpCas9 for in vitro transcription with Sp6
origin :
37
pi :
alt_names
SpCas9
hSpCas9
cloning
clone_methodRestriction Enzyme
cloning_site_3Xba I
cloning_site_5BamHI
promoterSP6
sequencing_primer_3T3
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namehumanized S.pyogenes Cas9
shRNA_sequence
size4272
species
tags
locationN terminal on insert
tag3xFLAG
plasmid copy :
Dr. Zhang produced the hSpCas9 gene, which has been deposited to Addgene (Plasmid 42230: pX330-U6-Chimeric_BB-CBh-hSpCas9 Cong et al. Science. 3 Jan 2013).
resistance markers :
1860
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA