This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
M4786 KanMX::hisG-URA3-HisG Disruptor Converter
catalog :
51690
citations: 1
Reference
Voth W, Jiang Y, Stillman D. New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Yeast. 2003;20:985-93 pubmed
product information
Catalog Number :
51690
Product Name :
M4786 KanMX::hisG-URA3-HisG Disruptor Converter
article :
doi10.1002/yea.1018
id7919
pubmed_id12898713
bacterial resistance :
Ampicillin
cloning :
backboneM4757 (kanMX::TRP1), Addgene plasmid #51687
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
Other
yeast marker swap
growth notes :
M4786 (kanMX::hisG-URA3-hisG) was made by digesting M4757 with BamHI and SalI and inserting a 3.9 kb BamHI SalI fragment with hisG URA3 hisG from plasmid M2524. The kanMX::hisG URA3 hisG converter is recyclable, so that any MX-based disruption can be reverted to an unmarked disruption, allowing reuse of markers.
growth strain :
Yeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisG
origin :
37
pi :
alt_names
URA3
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoter
sequencing_primer_3T7
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYEL021W
geneURA3
id856692
genbank_ids
mutation
namehisG-URA3-hisG
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
1853
tags :
Unknown
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA