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product summary
company name :
Addgene
product type :
cDNA
product name :
M4757 KanMX::TRP1 Disruptor Converter
catalog :
51687
citations: 1
| Reference |
|---|
Voth W, Jiang Y, Stillman D. New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Yeast. 2003;20:985-93 pubmed
|
product information
Catalog Number :
51687
Product Name :
M4757 KanMX::TRP1 Disruptor Converter
article :
| doi | 10.1002/yea.1018 |
| id | 7919 |
| pubmed_id | 12898713 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pAG25 (Goldstein and McCusker, 1999) | ||
| backbone_mutation | |||
| backbone_origin | |||
| backbone_size | |||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
First, plasmid pAG25 (Goldstein and McCusker, 1999) was digested with HindIII and PacI, treated with Klenow and ligated closed. This plasmid was digested with KpnI and SphI and the 0.9 kb KpnI SphI fragment containing TRP1 from pJJ246 was inserted, constructing M4757.
growth strain :
Yeast marker swap plasmid for converting KanMX3 to TRP1
origin :
37
pi :
|
resistance markers :
| 1853 |
tags :
Unknown
terms :
| TRP1 |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments