M2660 ura3::LYS2 Disruptor Converter | Addgene 51678 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

M2660 ura3::LYS2 Disruptor Converter

catalog :

51678

citations: 1

Reference
Voth W, Jiang Y, Stillman D. New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Yeast. 2003;20:985-93 pubmed

product information

Catalog Number :

51678

Product Name :

M2660 ura3::LYS2 Disruptor Converter

article :

doi	10.1002/yea.1018
id	7919
pubmed_id	12898713

bacterial resistance :

Ampicillin

cloning :

backbone

M581

backbone_mutation

backbone_origin

Marsh et al., 1984

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

Other

yeast marker swap

growth notes :

Plasmid M2660 (ura3::LYS2 ) was made by cutting M581 with EcoRV and PstI and replacing the 208 bp EcoRV PstI fragment with a 4.6 kb PvuII PstI fragment containing LYS2 from plasmid YDp-K.

growth strain :

Yeast marker swap plasmid for converting URA3 to LYS2

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	PstI
cloning_site_5	EcoRV
promoter
sequencing_primer_3	M13-R
sequencing_primer_5	M13-F20
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	YBR115C
gene	LYS2
id	852412

genbank_ids

mutation

name

LYS2

shRNA_sequence

size

species

4932

Saccharomyces cerevisiae