D771 leu2::LYS2 Disruptor Converter | Addgene 51677 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

D771 leu2::LYS2 Disruptor Converter

catalog :

51677

citations: 1

Reference
Voth W, Jiang Y, Stillman D. New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Yeast. 2003;20:985-93 pubmed

product information

Catalog Number :

51677

Product Name :

D771 leu2::LYS2 Disruptor Converter

article :

doi	10.1002/yea.1018
id	7919
pubmed_id	12898713

bacterial resistance :

Ampicillin

cloning :

backbone

pIC19H SXL2

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

Other

yeast marker swap

growth notes :

Plasmid D771 (leu2::LYS2) was constructed in two steps. First, plasmid YDp-K was cut with SalI and an EcoRI linker was inserted, constructing plasmid D764. Next, plasmid pIC19H SXL2 was cut with EcoRI and a 5 kb EcoRI fragment containing LYS2 from plasmid D764 was inserted, creating D771.

growth strain :

Yeast marker swap plasmid for converting LEU2 to LYS2

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	EcoRI
promoter
sequencing_primer_3	M13-R
sequencing_primer_5	M13-F20
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YBR115C
gene	LYS2
id	852412

genbank_ids

mutation

name

LYS2

shRNA_sequence

size

species

4932

Saccharomyces cerevisiae