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product summary
company name :
Addgene
product type :
cDNA
product name :
D588 lys2::HIS3 Disruptor Converter
catalog :
51675
citations: 1
| Reference |
|---|
Voth W, Jiang Y, Stillman D. New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Yeast. 2003;20:985-93 pubmed
|
product information
Catalog Number :
51675
Product Name :
D588 lys2::HIS3 Disruptor Converter
article :
| doi | 10.1002/yea.1018 |
| id | 7919 |
| pubmed_id | 12898713 |
bacterial resistance :
Ampicillin
cloning :
| backbone | YDp-K | |||
| backbone_mutation | ||||
| backbone_origin | ||||
| backbone_size | ||||
| promoter | ||||
| sequencing_primer_3 | ||||
| sequencing_primer_5 | ||||
| vector_types |
|
growth notes :
Plasmid D588 (lys2::HIS3 ) was constructed in two steps. First, plasmid YDp-K was cut with BglII and XhoI and ligated, deleting a 2477 bp fragment inside the LYS2 gene. This plasmid was then cleaved with BglII and a 1.2 kb BamHI fragment from YDp-H containing HIS3 was inserted, creating D558.
growth strain :
Yeast marker swap plasmid for converting LYS2 to HIS3
origin :
37
pi :
|
resistance markers :
| 1853 |
tags :
Unknown
terms :
| HIS3 |
| Other |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments