This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
M3499 ura3::ADE2 Disruptor Converter
catalog :
51674
citations: 2
Reference
Brink D, Borgström C, Tueros F, Gorwa Grauslund M. Real-time monitoring of the sugar sensing in Saccharomyces cerevisiae indicates endogenous mechanisms for xylose signaling. Microb Cell Fact. 2016;15:183 pubmed
Voth W, Jiang Y, Stillman D. New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Yeast. 2003;20:985-93 pubmed
product information
Catalog Number :
51674
Product Name :
M3499 ura3::ADE2 Disruptor Converter
article :
doi10.1002/yea.1018
id7919
pubmed_id12898713
bacterial resistance :
Ampicillin
cloning :
backbonepJJ244
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
Other
yeast marker swap
growth notes :
Plasmid M3499 (ura3::ADE2 ) was made by cutting pJJ244 with StuI and EcoRV and replacing the 248 bp StuI EcoRV fragment with a 3.6 kb BamHI blunted with Klenow) fragment containing ADE2 from plasmid M1144.
growth strain :
Yeast marker swap plasmid for converting URA3 to ADE2
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRV
cloning_site_5StuI
promoterADE2
sequencing_primer_3M13R
sequencing_primer_5M13-F20
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesYOR128C
geneADE2
id854295
genbank_ids
mutation
nameADE2
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
resistance markers :
1853
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA