This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pREDTKI
catalog :
51628
citations: 4
Reference
Shipley A, Frampton G, Davies B, Umlauf B. Generating Shigella that internalize into glioblastoma cells. Front Oncol. 2023;13:1229747 pubmed publisher
Bourgeois J, Anderson C, Wang L, Modliszewski J, Chen W, Schott B, et al. Integration of the Salmonella Typhimurium Methylome and Transcriptome Reveals That DNA Methylation and Transcriptional Regulation Are Largely Decoupled under Virulence-Related Conditions. MBio. 2022;13:e0346421 pubmed publisher
Moxley W, Eiteman M. Pyruvate Production by Escherichia coli by Use of Pyruvate Dehydrogenase Variants. Appl Environ Microbiol. 2021;87:e0048721 pubmed publisher
Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, et al. High-efficiency scarless genetic modification in Escherichia coli by using lambda red recombination and I-SceI cleavage. Appl Environ Microbiol. 2014;80:3826-34 pubmed publisher
product information
Catalog Number :
51628
Product Name :
pREDTKI
article :
doi10.1128/AEM.00313-14
id8801
pubmed_id24747889
bacterial resistance :
Kanamycin
cloning :
backbonepKD46
backbone_mutationreplace amp to kan
backbone_origin
backbone_size6300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Other
Red recombinase and I-SceI endonuclease
growth strain :
E coli Genetic Modification
growth temp :
Culture the plate in 30 C or 28 C for 24h or up to 48h to see if any colonies appear. LB +1% glucose plate, or SOC plate improve growth.
origin :
30
pi :
alt_names
mitochondrial intron of Saccharomyces cerevisiae
cloning
clone_methodRestriction Enzyme
cloning_site_3NcoI
cloning_site_5NcoI
promotertrc
sequencing_primer_3
sequencing_primer_5none
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesQ0160
geneSCEI
id854590
genbank_ids
mutation
nameI-SceI
shRNA_sequence
size3000
species
4932
Saccharomyces cerevisiae
tags
plasmid copy :
pUC19RP12 Nucl. Acids Res. (1999) 27 (22): 4409-4415. Gy rgy P sfai, Institute of Biochemistry, Biological Research Center, H-6701 Szeged, Hungary
resistance markers :
1850
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA