This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pREDTAI
catalog :
51627
citations: 1
Reference
Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, et al. High-efficiency scarless genetic modification in Escherichia coli by using lambda red recombination and I-SceI cleavage. Appl Environ Microbiol. 2014;80:3826-34 pubmed publisher
product information
Catalog Number :
51627
Product Name :
pREDTAI
article :
doi10.1128/AEM.00313-14
id8801
pubmed_id24747889
bacterial resistance :
Ampicillin
cloning :
backbonepKD46
backbone_mutation
backbone_origin
backbone_size6300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Other
Red recombinase and I-SceI endonuclease
growth strain :
E coli Genetic Modification
growth temp :
Culture the plate in 30 C or 28 C for 24h or up to 48h to see if any colonies appear. LB +1% glucose plate, or SOC plate improve growth.
origin :
30
pi :
alt_names
mitochondrial intron of Saccharomyces cerevisiae
cloning
clone_methodRestriction Enzyme
cloning_site_3NcoI
cloning_site_5NcoI
promotertrc
sequencing_primer_3
sequencing_primer_5none
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesQ0160
geneSCEI
id854590
genbank_ids
mutation
nameI-SceI
shRNA_sequence
size3000
species
4932
Saccharomyces cerevisiae
tags
plasmid copy :
pUC19RP12 Nucl. Acids Res. (1999) 27 (22): 4409-4415. Gy rgy P sfai, Institute of Biochemistry, Biological Research Center, H-6701 Szeged, Hungary
resistance markers :
1850
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA