pME-nlsGal80(opt) | Addgene 51618 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pME-nlsGal80(opt)

catalog :

51618

citations: 1

Reference
Fujimoto E, Gaynes B, Brimley C, Chien C, Bonkowsky J. Gal80 intersectional regulation of cell-type specific expression in vertebrates. Dev Dyn. 2011;240:2324-34 pubmed publisher

product information

Catalog Number :

51618

Product Name :

pME-nlsGal80(opt)

article :

doi	10.1002/dvdy.22734
id	7910
pubmed_id	21905164

bacterial resistance :

Kanamycin

cloning :

backbone

Gateway middle entry clone (pME)

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

vertebrate expression

growth notes :

for the nuclear localization signal (Kwan et al., 2007) and 5'-CAGTGAGTAGTGAGAGATATTTG-3' for codon-optimized Gal80 (GenScript; GenBank accession no. JN314417).
5'-ATGGCTCCAAAGAAGAAGCGTAAGGTAATGGACTAC
AACAAAAGGAGCAG-3'
pME-NLS-Gal80opt was generated by PCR using primers

growth strain :

vertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stop

origin :

pi :

alt_names

Gal80

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	M13R
sequencing_primer_5	M13-F20
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	YML051W
gene	GAL80
id	854954

genbank_ids

mutation

codon optimized

name

Gal80(opt)

shRNA_sequence

size

species

4932

Saccharomyces cerevisiae