This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-myc-CD4-Sema4D
catalog :
51603
citations: 1
Reference
Raissi A, Staudenmaier E, David S, Hu L, Paradis S. Sema4D localizes to synapses and regulates GABAergic synapse development as a membrane-bound molecule in the mammalian hippocampus. Mol Cell Neurosci. 2013;57:23-32 pubmed publisher
product information
Catalog Number :
51603
Product Name :
pCMV-myc-CD4-Sema4D
article :
doi10.1016/j.mcn.2013.08.004
id7907
pubmed_id24036351
bacterial resistance :
Ampicillin
cloning :
backbonepCMV
backbone_mutation
backbone_origin
backbone_size4000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that the sequence of the myc epitope tag in this plasmid is MQKLISEEDLL, which differs from the standard EQKLISEEDLL. The CD4 insert in this plasmid contains N64I and K385E mutations when compared to GenBank reference sequence NP_038688.2.
growth strain :
expresses Sema4D/CD4 chimera with intracellular Sema4D, extracellular CD4
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BglII
promoterCMV
sequencing_primer_3TACAACTGCTACAAGGGCTAC
sequencing_primer_5gagacacggagagggtcttc
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationSema4D amino acids 1-754 have been replaced with CD4 amino acids 26-425.
nameCD4-Sema4D
shRNA_sequence
size1700
species
10090
Mus musculus
tags
locationN terminal on backbone
tagmyc
resistance markers :
1856
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA