This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-myc-Rem2 S334A
catalog :
51593
citations: 1
Reference
Ghiretti A, Kenny K, Marr M, Paradis S. CaMKII-dependent phosphorylation of the GTPase Rem2 is required to restrict dendritic complexity. J Neurosci. 2013;33:6504-15 pubmed publisher
product information
Catalog Number :
51593
Product Name :
pCMV-myc-Rem2 S334A
article :
doi10.1523/JNEUROSCI.3861-12.2013
id7905
pubmed_id23575848
bacterial resistance :
Ampicillin
cloning :
backbonepCMV
backbone_mutation
backbone_origin
backbone_size4000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Please note that the sequence of the myc epitope tag in this plasmid is MQKLISEEDLL, which differs from the standard EQKLISEEDLL. The Rem2 insert in this plasmid contains T13I and T15I mutations when compared to GenBank reference sequence NP_542764.2.
growth strain :
expresses RNAiR myc-tagged Rem2 S-A mutant
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3CATTCTAGTTGTGGTTTGTCC
sequencing_primer_5GATCCGGTACTAGAGGAACTGAAAAAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAW411893
geneRem2
id140743
genbank_ids
mutationSerine 334 to Alanine; also RNAi resistant
nameRem2
shRNA_sequence
size1000
species
10090
Mus musculus
tags
locationN terminal on backbone
tagmyc
resistance markers :
1856
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA