PhyB-mCherry-MYO1 | Addgene 51570 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

PhyB-mCherry-MYO1

catalog :

51570

citations: 2

Reference
Nakamura K, Sawada K, Kinose Y, Yoshimura A, Toda A, Nakatsuka E, et al. Exosomes Promote Ovarian Cancer Cell Invasion through Transfer of CD44 to Peritoneal Mesothelial Cells. Mol Cancer Res. 2017;15:78-92 pubmed publisher
Yang X, Jost A, Weiner O, Tang C. A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast. Mol Biol Cell. 2013;24:2419-30 pubmed publisher

product information

Catalog Number :

51570

Product Name :

PhyB-mCherry-MYO1

article :

doi	10.1091/mbc.E13-03-0126
id	7902
pubmed_id	23761071

bacterial resistance :

Ampicillin

cloning :

backbone

pRS305

backbone_mutation

backbone_origin

backbone_size

7236

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Yeast Expression

growth notes :

There is a 15-aa linker (EFDSAGSAGSAGGSS) between the PhyB and mCherry and a 10-aa linker (SAGSAGKASG) between mCherry and anchor gene.

growth strain :

Constitutively express myosin ring localized PhyB in Saccharomyces cerevisiae

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	Apa1
promoter	ADH1pr
sequencing_primer_3
sequencing_primer_5	mCherry F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B
gene	PHYB
id	816394

genbank_ids

mutation

1-908aa only

name

PhyB

shRNA_sequence

size

10819

species

3702

Arabidopsis thaliana