This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PhyB-mCherry-CAAX
catalog :
51567
citations: 2
Reference
Yasunaga T, Wiegel J, Bergen M, Helmstädter M, Epting D, Paolini A, et al. Microridge-like structures anchor motile cilia. Nat Commun. 2022;13:2056 pubmed publisher
Yang X, Jost A, Weiner O, Tang C. A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast. Mol Biol Cell. 2013;24:2419-30 pubmed publisher
product information
Catalog Number :
51567
Product Name :
PhyB-mCherry-CAAX
article :
doi10.1091/mbc.E13-03-0126
id7902
pubmed_id23761071
bacterial resistance :
Ampicillin
cloning :
backbonepRS305
backbone_mutation
backbone_origin
backbone_size7236
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
There is a 15-aa linker (EFDSAGSAGSAGGSS) between the PhyB and mCherry and a 10-aa linker (SAGSAGKASG) between mCherry and anchor gene.
growth strain :
Constitutively express PM (Plasma membrane) localized PhyB in Saccharomyces cerevisiae
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5Apa1
promoterADH1pr
sequencing_primer_3
sequencing_primer_5mCherry F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B
genePHYB
id816394
genbank_ids
mutation1-908aa only
namePhyB
shRNA_sequence
size5101
species
3702
Arabidopsis thaliana
tags
locationC terminal on insert
tagmCherry
locationC terminal on insert
tagCAAX
plasmid copy :
Jared Toettcher
resistance markers :
1848
tags :
High Copy
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA