This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGL3Basic-ME.2/ApoEpromoter
catalog :
51436
citations: 2
Reference
Fernandes G, Bocco B, Fonseca T, McAninch E, Jo S, Lartey L, et al. The Foxo1-Inducible Transcriptional Repressor Zfp125 Causes Hepatic Steatosis and Hypercholesterolemia. Cell Rep. 2018;22:523-534 pubmed publisher
Pencheva N, Buss C, Posada J, Merghoub T, Tavazoie S. Broad-spectrum therapeutic suppression of metastatic melanoma through nuclear hormone receptor activation. Cell. 2014;156:986-1001 pubmed publisher
product information
Catalog Number :
51436
Product Name :
pGL3Basic-ME.2/ApoEpromoter
article :
doi10.1016/j.cell.2014.01.038
id7861
pubmed_id24581497
bacterial resistance :
Ampicillin
cloning :
backbonepGL3 Basic
backbone_mutation
backbone_originPromega
backbone_size4818
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth strain :
This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancer
origin :
37
pi :
alt_names
ME2 malic enzyme 2
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5MluI
promoter
sequencing_primer_3CTTTATGTTTTTGGCGTCTTCCA
sequencing_primer_5CTAGCAAAATAGGCTGTCCC
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAD2, APO-E, ApoE4, LDLCQ5, LPG
geneAPOE
id348
aliasesODS1
geneME2
id4200
genbank_ids
mutationThe ME.2 enhancer is fused upstream of the ApoE gene promoter (-980/+93 bp)
nameAPOE apolipoprotein E
shRNA_sequence
size1698
species
9606
Homo sapiens
tags
resistance markers :
1411
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA