This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGolden-Neo
catalog :
51422
citations: 3
Reference
Schertzer M, Braceros K, Starmer J, Cherney R, Lee D, Salazar G, et al. lncRNA-Induced Spread of Polycomb Controlled by Genome Architecture, RNA Abundance, and CpG Island DNA. Mol Cell. 2019;: pubmed publisher
Sternburg E, Dias K, Karginov F. Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells. J Vis Exp. 2017;: pubmed publisher
Luo Y, Lin L, Bolund L, Sørensen C. Efficient construction of rAAV-based gene targeting vectors by Golden Gate cloning. Biotechniques. 2014;56:263-8 pubmed publisher
product information
Catalog Number :
51422
Product Name :
pGolden-Neo
article :
doi10.2144/000114169
id9450
pubmed_id24806227
bacterial resistance :
Kanamycin
cloning :
backbonepTopo
backbone_mutationno
backbone_originlifetechnologies
backbone_size3519
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Contain one loxP-PGK-Neo-pA-loxP cassette, used for golden-gate reaction
origin :
37
pi :
alt_names
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoterPGK
sequencing_primer_3GTAAAACGACGGCCAGT
sequencing_primer_5GGAAACAGCTATGACCATG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHEL-S-68p, MIG10, PGKA
genePGK1
id5230
genbank_ids
mutation
namePGK1 phosphoglycerate kinase 1
shRNA_sequence
size1737
species
9606
Homo sapiens
tags
plasmid copy :
The PGK-Neo-pA cassette was PCR amplified from the pNeDaKo-Neo vector from Bert Vogelstein and Kenneth W. Kinzler, John Hopkins University, Baltimore, US
resistance markers :
1844
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA