This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAM1
catalog :
51401
citations: 1
Reference
Mahdavi A, Szychowski J, Ngo J, Sweredoski M, Graham R, Hess S, et al. Identification of secreted bacterial proteins by noncanonical amino acid tagging. Proc Natl Acad Sci U S A. 2014;111:433-8 pubmed publisher
product information
Catalog Number :
51401
Product Name :
pAM1
article :
doi10.1073/pnas.1301740111
id7848
pubmed_id24347637
bacterial resistance :
Kanamycin
cloning :
backbonepQE80 kan
backbone_mutationInsert containing the MetG promoter was inserted into the Nhe1 cut site in the backbone of the pQE80 vector.
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
growth strain :
Expresses truncated version of NLL-MetRS from endogenous MetG promoter
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Nhe1
cloning_site_5Nhe1
promoterMetG
sequencing_primer_3T0term-R
sequencing_primer_53primeUP
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesb2114, ECK2107, JW2101
genemetG
id946643
genbank_ids
mutationL13N, Y260L, H301L; Please note that a truncated version of MetRS is used for pAM1. The extra stop codon was deliberately put in the sequence and shortens the sequence from 677AA to 548AA.
nameMetRS
shRNA_sequence
size
species
32644
Other
E coli
tags
resistance markers :
1834
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA