pGL3-CMV-Cdc42 3'UTR mutant (position 577-599 and 997-1019)

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product summary

company name :

Addgene

product type :

cDNA

product name :

pGL3-CMV-Cdc42 3'UTR mutant (position 577-599 and 997-1019)

catalog :

51390

citations: 1

Reference
Park S, Lee J, Ha M, Nam J, Kim V. miR-29 miRNAs activate p53 by targeting p85 alpha and CDC42. Nat Struct Mol Biol. 2009;16:23-9 pubmed publisher

product information

Catalog Number :

51390

Product Name :

pGL3-CMV-Cdc42 3'UTR mutant (position 577-599 and 997-1019)

article :

doi	10.1038/nsmb.1533
id	7836
pubmed_id	19079265

bacterial resistance :

Ampicillin

cloning :

backbone

Cdc42 3'UTR

backbone_mutation

backbone_origin

Promega (modified to have 3' multi cloning sites)

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Luciferase

growth notes :

miR-29 seed mutant (position: 578-584, 997-1003, TGCT->GTAG)

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	XbaI
promoter	CMV
sequencing_primer_3	EBV_rev_primer
sequencing_primer_5	Luc-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CDC42Hs, G25K, TKS
gene	CDC42
id	998

genbank_ids

mutation

miR-29 binding site mutant (position 577-599, 997-1019)

name

NM_001039802.1

shRNA_sequence

size

species

9606

Homo sapiens