pGL3-23P639 | Addgene 51388 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGL3-23P639

catalog :

51388

citations: 3

Reference
Lu Y, Chan Y, Wu J, Feng Z, Yuan H, Li Q, et al. CRISPR/Cas9 screens unravel miR-3689a-3p regulating sorafenib resistance in hepatocellular carcinoma via suppressing CCS/SOD1-dependent mitochondrial oxidative stress. Drug Resist Updat. 2023;71:101015 pubmed publisher
Lu Y, Chan Y, Tan H, Zhang C, Guo W, Xu Y, et al. Epigenetic regulation of ferroptosis via ETS1/miR-23a-3p/ACSL4 axis mediates sorafenib resistance in human hepatocellular carcinoma. J Exp Clin Cancer Res. 2022;41:3 pubmed publisher
Lee Y, Kim M, Han J, Yeom K, Lee S, Baek S, et al. MicroRNA genes are transcribed by RNA polymerase II. EMBO J. 2004;23:4051-60 pubmed

product information

Catalog Number :

51388

Product Name :

pGL3-23P639

article :

doi	10.1038/sj.emboj.7600385
id	7838
pubmed_id	15372072

bacterial resistance :

Ampicillin

cloning :

backbone

pGL3-basic

backbone_mutation

backbone_origin

Promega

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Luciferase

growth notes :

position: -603~+36nt (compared to transcription start site of pri-miR-23a) (sequence: Fig. 4A) Addgene sequencing has found a T50260C and A50093 insert relative to human chromosome 19 (gb AC020916.8), this is in the promoter region and is not believed to effect the function of the plasmid.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	KpnI
promoter
sequencing_primer_3	LucNrev_primer
sequencing_primer_5	RVprimer3_primer
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	MIRN23A, hsa-mir-23a, miRNA23A, mir-23a
gene	MIR23A
id	407010

genbank_ids

mutation

name

miR-23a promoter region

shRNA_sequence

size

species

9606

Homo sapiens