This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1-FLAG-Dicer-5'-pocket mutant
catalog :
51368
citations: 1
Reference
Park J, Heo I, Tian Y, Simanshu D, Chang H, Jee D, et al. Dicer recognizes the 5' end of RNA for efficient and accurate processing. Nature. 2011;475:201-5 pubmed publisher
product information
Catalog Number :
51368
Product Name :
pcDNA3.1-FLAG-Dicer-5'-pocket mutant
article :
doi10.1038/nature10198
id7832
pubmed_id21753850
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originInvitrogen
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
origin :
37
pi :
alt_names
Dicer1
cloning
clone_methodRestriction Enzyme
cloning_site_3ApaI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3BGH_rev_primer
sequencing_primer_5CMV_fwd_primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, aviD
geneDICER1
id23405
genbank_ids
mutation5' pocket mutant (R778A, R780A, R811A, H982A, R986A, R993A)
nameDicer
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG
plasmid copy :
Note that the full-length wild type Dicer was cloned by Dr. Patrick Provost (PMID: 12411504).
resistance markers :
1833
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA