This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCS2-3xFLAG-NLS-SpCas9-NLS
catalog :
51307
citations: 16
Reference
Perillo M, Swartz S, Pieplow C, Wessel G. Molecular mechanisms of tubulogenesis revealed in the sea star hydro-vascular organ. Nat Commun. 2023;14:2402 pubmed publisher
Liu X, Yang J, Li Z, Liu R, Wu X, Zhang Z, et al. YIPF5 (p.W218R) mutation induced primary microcephaly in rabbits. Neurobiol Dis. 2023;182:106135 pubmed publisher
Parain K, Lourdel S, Donval A, Chesneau A, Borday C, Bronchain O, et al. CRISPR/Cas9-Mediated Models of Retinitis Pigmentosa Reveal Differential Proliferative Response of Müller Cells between Xenopus laevis and Xenopus tropicalis. Cells. 2022;11: pubmed publisher
Brun N, Salanga M, Mora Zamorano F, Lamb D, Goldstone J, Stegeman J. Orphan cytochrome P450 20a1 CRISPR/Cas9 mutants and neurobehavioral phenotypes in zebrafish. Sci Rep. 2021;11:23892 pubmed publisher
Wessel G, Wada Y, Yajima M, Kiyomoto M. Sperm lacking Bindin are infertile but are otherwise indistinguishable from wildtype sperm. Sci Rep. 2021;11:21583 pubmed publisher
Dalgin G, Prince V. Midline morphogenesis of zebrafish foregut endoderm is dependent on Hoxb5b. Dev Biol. 2021;471:1-9 pubmed publisher
Wessel G, Kiyomoto M, Shen T, Yajima M. Genetic manipulation of the pigment pathway in a sea urchin reveals distinct lineage commitment prior to metamorphosis in the bilateral to radial body plan transition. Sci Rep. 2020;10:1973 pubmed publisher
Salanga M, Brun N, Francolini R, Stegeman J, Goldstone J. CRISPR-Cas9-Mutated Pregnane X Receptor (pxr) Retains Pregnenolone-induced Expression of cyp3a65 in Zebrafish (Danio rerio) Larvae. Toxicol Sci. 2020;174:51-62 pubmed publisher
Song Y, Sui T, Zhang Y, Wang Y, Chen M, Deng J, et al. Genetic deletion of a short fragment of glucokinase in rabbit by CRISPR/Cas9 leading to hyperglycemia and other typical features seen in MODY-2. Cell Mol Life Sci. 2019;: pubmed publisher
Song Y, Xu Y, Liang M, Zhang Y, Chen M, Deng J, et al. CRISPR/Cas9-mediated mosaic mutation of SRY gene induces hermaphroditism in rabbits. Biosci Rep. 2018;38: pubmed publisher
Shevidi S, Uchida A, Schudrowitz N, Wessel G, Yajima M. Single nucleotide editing without DNA cleavage using CRISPR/Cas9-deaminase in the sea urchin embryo. Dev Dyn. 2017;246:1036-1046 pubmed publisher
Yuan L, Yao H, Xu Y, Chen M, Deng J, Song Y, et al. CRISPR/Cas9-Mediated Mutation of ?A-Crystallin Gene Induces Congenital Cataracts in Rabbits. Invest Ophthalmol Vis Sci. 2017;58:BIO34-BIO41 pubmed publisher
Song Y, Liu T, Wang Y, Deng J, Chen M, Yuan L, et al. Mutation of the Sp1 binding site in the 5' flanking region of SRY causes sex reversal in rabbits. Oncotarget. 2017;8:38176-38183 pubmed publisher
Oulhen N, Swartz S, Laird J, Mascaro A, Wessel G. Transient translational quiescence in primordial germ cells. Development. 2017;144:1201-1210 pubmed publisher
Oulhen N, Wessel G. Albinism as a visual, in vivo guide for CRISPR/Cas9 functionality in the sea urchin embryo. Mol Reprod Dev. 2016;83:1046-1047 pubmed publisher
Guo X, Zhang T, Hu Z, Zhang Y, Shi Z, Wang Q, et al. Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis. Development. 2014;141:707-14 pubmed publisher
product information
Catalog Number :
51307
Product Name :
pCS2-3xFLAG-NLS-SpCas9-NLS
article :
doi10.1242/dev.099853
id7842
pubmed_id24401372
bacterial resistance :
Ampicillin
cloning :
backbonepCS2+
backbone_mutation
backbone_origin
backbone_size4095
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
Other
Xenopus tropicalis expression
growth strain :
Cas9 expression plasmid
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3T3
sequencing_primer_5SP6
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
name3xFLAG-NLS-SpCas9-NLS
shRNA_sequence
size4272
species
tags
locationN terminal on insert
tag3x Flag
locationN terminal on insert
tagNLS
locationC terminal on insert
tagNLS
resistance markers :
1832
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA