This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti.tie1.H2B.eYFP.W
catalog :
51293
citations: 1
Reference
Sato Y, Poynter G, Huss D, Filla M, Czirok A, Rongish B, et al. Dynamic analysis of vascular morphogenesis using transgenic quail embryos. PLoS ONE. 2010;5:e12674 pubmed publisher
product information
Catalog Number :
51293
Product Name :
pLenti.tie1.H2B.eYFP.W
article :
doi10.1371/journal.pone.0012674
id6565
pubmed_id20856866
bacterial resistance :
Ampicillin
cloning :
backbonepRRLsin.cPPT.Tie1p.eGFP.wpre
backbone_mutation
backbone_originLuigi Naldini
backbone_size6697
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth notes :
Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.
growth strain :
Lentivector that encodes H2B-eYFP under the transcriptional control of the mouse TIE1 promoter
origin :
37
pi :
alt_names
Histone 2B-enhanced yellow fluorescent protein
cloning
clone_methodRestriction Enzyme
cloning_site_3BsrGI
cloning_site_5AgeI
promotermTie1
sequencing_primer_3not known
sequencing_primer_5not known
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesGL105, H2B, H2B-GL105, H2B.1, H2BE, H2BFQ, H2BGL105, H2BQ, HIST2H2BE
geneH2BC21
id8349
genbank_ids
mutation
nameH2B-eYFP
shRNA_sequence
size1116
species
9606
Homo sapiens
tags
locationC terminal on insert
tagH2B-eYFP
plasmid copy :
Original plasmid was a kind gift from Dr. Luigi Naldini
resistance markers :
1512
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA