This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pU6-neo-TET
catalog :
51286
citations: 1
Reference
Kroutter E, Belancio V, Wagstaff B, Roy Engel A. The RNA polymerase dictates ORF1 requirement and timing of LINE and SINE retrotransposition. PLoS Genet. 2009;5:e1000458 pubmed publisher
product information
Catalog Number :
51286
Product Name :
pU6-neo-TET
article :
doi10.1371/journal.pgen.1000458
id7806
pubmed_id19390602
bacterial resistance :
Ampicillin
cloning :
backbonepAluYa5-neo-TET
backbone_mutation
backbone_originAddgene plasmid 51283
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
U6 is tagged with the self splicing intron neo cassette such that G418 resistance will be obtained only if retrotransposition occurs. Please note that the cassette is in the reverse orientation within the MCS relative to the full sequence--This has no functional consequence.
growth strain :
expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette
origin :
37
pi :
alt_names
HUMUG6
cloning
clone_methodRestriction Enzyme
cloning_site_3AatI
cloning_site_5BamHI
promoterU6 upstream enhancer sequence
sequencing_primer_3SV40pro-F
sequencing_primer_5M13-pUC-Fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRNU6, RNU6A, U6, U6-1
geneRNU6-1
id26827
genbank_ids
mutation
nameU6
shRNA_sequence
size512
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagneoTET cassette with a tetrahymena self-splicing intron interrupting the neo gene
resistance markers :
1826
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA