p7SL-neo-TET | Addgene 51285 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

p7SL-neo-TET

catalog :

51285

citations: 1

Reference
Kroutter E, Belancio V, Wagstaff B, Roy Engel A. The RNA polymerase dictates ORF1 requirement and timing of LINE and SINE retrotransposition. PLoS Genet. 2009;5:e1000458 pubmed publisher

product information

Catalog Number :

51285

Product Name :

p7SL-neo-TET

article :

doi	10.1371/journal.pgen.1000458
id	7806
pubmed_id	19390602

bacterial resistance :

Ampicillin

cloning :

backbone

pAluYa5-neo-TET

backbone_mutation

backbone_origin

Addgene plasmid 51283

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

7SL is tagged with the self splicing intron neo cassette such that G418 resistance will be obtained only if retrotransposition occurs. Primers used for amplification of 7SL: GGATCCGCCCAGTGTGGGTGTGTCC GACGTCAAGAGACGGGGTCTCGCTATG Please note that the cassette is in the reverse orientation within the MCS relative to the full sequence--This has no functional consequence.

growth strain :

expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	AatII
cloning_site_5	BamHI
promoter	7SL upstream pollII enhancer
sequencing_primer_3	SV40pro-F
sequencing_primer_5	M13-pUC-Fwd
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	7L1C, 7L30.1, 7SL1c, RNSRP2
gene	RN7SL2
id	378706

genbank_ids

M20910

mutation

name

7SL RNA

shRNA_sequence

size

413

species

9606

Homo sapiens