pmRFP-N1 human cofilin R21Q | Addgene 51279 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pmRFP-N1 human cofilin R21Q

catalog :

51279

citations: 3

Reference
Minamide L, Hylton R, Swulius M, Bamburg J. Visualizing Cofilin-Actin Filaments by Immunofluorescence and CryoEM: Essential Steps for Observing Cofilactin in Cells. Methods Mol Biol. 2023;2593:265-281 pubmed publisher
Pearce K, Bell M, Linthicum W, Wen Q, Srinivasan J, Rangamani P, et al. Gαq-mediated calcium dynamics and membrane tension modulate neurite plasticity. Mol Biol Cell. 2019;:mbcE19090536 pubmed publisher
Mi J, Shaw A, Pak C, Walsh K, Minamide L, Bernstein B, et al. A genetically encoded reporter for real-time imaging of cofilin-actin rods in living neurons. PLoS ONE. 2013;8:e83609 pubmed publisher

product information

Catalog Number :

51279

Product Name :

pmRFP-N1 human cofilin R21Q

article :

doi
id	7815
pubmed_id	24391794

bacterial resistance :

Kanamycin

cloning :

backbone

pmRFP-N1

backbone_mutation

backbone_origin

R.Y. Tsien, PNAS 2002 v99 pp7877 7882

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

This cofilin plasmid contains some silent mutations to make it resistant to an siRNA used in the Bamburg lab. The mutations change the wildtype sequence (AAG TCT TCA ACG CCA GAG GAG) into AAG AGC TCA ACC CCA GAG GAG.

growth strain :

expresses human cofilin R21Q mutant fused to mRFP in mammalian cells

origin :

pi :

alt_names

CFL1

cloning

clone_method	Restriction Enzyme
cloning_site_3	XmaI
cloning_site_5	EcoRI
promoter	CMV
sequencing_primer_3	mRFP1-R
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CFL, HEL-S-15, cofilin
gene	CFL1
id	1072

genbank_ids

mutation

R21Q, non-rod-forming mutation that can be used to track cofilin-actin rods in vivo without causing cofilin overexpression artifacts

name

cofilin 1

shRNA_sequence

size

500

species

9606

Homo sapiens