This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pST1374-N-NLS-flag-linker-Cas9-H840A
catalog :
51129
citations: 4
Reference
Jiang W, Feng S, Huang S, Yu W, Li G, Yang G, et al. BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity. Cell Res. 2018;28:855-861 pubmed publisher
Gopalappa R, Suresh B, Ramakrishna S, Kim H. Paired D10A Cas9 nickases are sometimes more efficient than individual nucleases for gene disruption. Nucleic Acids Res. 2018;46:e71 pubmed publisher
Lei L, Chen H, Xue W, Yang B, Hu B, Wei J, et al. APOBEC3 induces mutations during repair of CRISPR-Cas9-generated DNA breaks. Nat Struct Mol Biol. 2018;25:45-52 pubmed publisher
Shen B, Zhang W, Zhang J, Zhou J, Wang J, Chen L, et al. Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Nat Methods. 2014;11:399-402 pubmed publisher
product information
Catalog Number :
51129
Product Name :
pST1374-N-NLS-flag-linker-Cas9-H840A
article :
doi10.1038/nmeth.2857
id8173
pubmed_id24584192
bacterial resistance :
Ampicillin
cloning :
backboneAddgene 44758
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
CRISPR
growth strain :
Cas9 nickase optimized for nuclear import.
origin :
37
pi :
alt_names
Cas9 H840A
cloning
clone_methodRestriction Enzyme
cloning_site_3ApaI
cloning_site_5NheI
promoterCMV
sequencing_primer_3bGH-pA-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationH840A
nameCas9 nickase
shRNA_sequence
size4263
species
tags
locationN terminal on insert
tagNLS
locationN terminal on insert
tagFlag
resistance markers :
1511
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA