This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pACT2-CAS9c
catalog :
51055
citations: 1
Reference
Gao Y, Zhao Y. Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing. J Integr Plant Biol. 2014;56:343-9 pubmed publisher
product information
Catalog Number :
51055
Product Name :
pACT2-CAS9c
article :
doi10.1111/jipb.12152
id7771
pubmed_id24373158
bacterial resistance :
Ampicillin
cloning :
backbonepACT2
backbone_mutationSequences between the two HindIII sites are replaced by Cas9c. The GAL4 activation domain in the original vector was removed.
backbone_originClonetech
backbone_size8117
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
Yeast Expression
CRISPR
growth strain :
Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.
origin :
37
pi :
alt_names
Cas9
hCas9
hCas9c
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoteryeast ADH1 promoter
sequencing_primer_3ACGTATCTACCAACGATTTGACC
sequencing_primer_5CCTCGTCATTGTTCTCGTTCC
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameCas9c
shRNA_sequence
size4140
species
100
Synthetic
tags
locationC terminal on insert
tagSV40 NLS
plasmid copy :
Luhan Yang from George M. Church Laboratory in Harvard Medical School.
resistance markers :
1817
tags :
High Copy
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA