This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-N2-6MYC-hMID2
catalog :
51035
citations: 1
| Reference |
|---|
Short K, Hopwood B, Yi Z, Cox T. MID1 and MID2 homo- and heterodimerise to tether the rapamycin-sensitive PP2A regulatory subunit, alpha 4, to microtubules: implications for the clinical variability of X-linked Opitz GBBB syndrome and other developmental disorders. BMC Cell Biol. 2002;3:1 pubmed
|
product information
Catalog Number :
51035
Product Name :
pCMV-N2-6MYC-hMID2
article :
| doi | 10.1186/1471-2121-3-1 |
| id | 7762 |
| pubmed_id | 11806752 |
bacterial resistance :
Kanamycin
cloning :
| backbone | pCMV-N2-6Myc | |
| backbone_mutation | Vector is modified from the pEGFP-N2 vector (Clontech, Palo Alto, CA). Briefly, the GFP coding region was excised from pEGFP-N2 with NotI and BamHI, the 5' overhangs filled using T4 DNA polymerase and the vector religated to give pCMV-N2. Six copies of the myc epitope containing a start codon was amplified from pGEM-6mycT (gift from M. Whitelaw, University of Adelaide), and cloned into the HindIII site of pCMV-N2. | |
| backbone_origin | Cox lab | |
| backbone_size | ||
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
Please note: hMID2 begins at residue 19 of the NCBI reference sequence.
growth strain :
mammalian expression of myc-tagged human MID2
origin :
37
pi :
|
resistance markers :
| 1815 |
tags :
Unknown
terms :
| Neomycin (select with G418) |
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments