This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSLQ1661-sgMUC4-E3(F+E)
catalog :
51025
citations: 7
Reference
Castells Garcia A, Ed Daoui I, González Almela E, Vicario C, Ottestrom J, Lakadamyali M, et al. Super resolution microscopy reveals how elongating RNA polymerase II and nascent RNA interact with nucleosome clutches. Nucleic Acids Res. 2021;: pubmed publisher
Athmane N, Williamson I, Boyle S, Biddie S, Bickmore W. MUC4 is not expressed in cell lines used for live cell imaging. Wellcome Open Res. 2021;6:265 pubmed publisher
Mao S, Ying Y, Wu X, Krueger C, Chen A. CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci. Nucleic Acids Res. 2019;47:e131 pubmed publisher
Neguembor M, Sebastian Perez R, Aulicino F, Gomez Garcia P, Cosma M, Lakadamyali M. (Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes. Nucleic Acids Res. 2018;46:e30 pubmed publisher
Qin P, Parlak M, Kuscu C, Bandaria J, Mir M, Szlachta K, et al. Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9. Nat Commun. 2017;8:14725 pubmed publisher
Fu Y, Rocha P, Luo V, Raviram R, Deng Y, Mazzoni E, et al. CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci. Nat Commun. 2016;7:11707 pubmed publisher
Chen B, Gilbert L, Cimini B, Schnitzbauer J, Zhang W, Li G, et al. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell. 2013;155:1479-91 pubmed publisher
product information
Catalog Number :
51025
Product Name :
pSLQ1661-sgMUC4-E3(F+E)
article :
doi10.1016/j.cell.2013.12.001
id7755
pubmed_id24360272
bacterial resistance :
Ampicillin
cloning :
backbonepSico
backbone_mutationNone
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth notes :
gRNA target sequence GTGGCGTGACCTGTGGATGCTG
growth strain :
Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting the repetitive sequence of human MUC4 exon 3
growth temp :
The depositing lab suggests using Stellar (Clontech) cells for growth and maximal DNA prep yield. The plasmid is provided in NEB Stable cells to reduce the chance of recombination.
origin :
37
pi :
alt_names
sgRNA(F+E)
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5BstXI
promotermouse U6
sequencing_primer_3atgcatggcggtaatacggttatc
sequencing_primer_5gacgcgccatctctaggcc
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesASGP, HSA276359, MUC-4
geneMUC4
id4585
genbank_ids
mutation
nameoptimized sgRNA
shRNA_sequence
size115
species
9606
Homo sapiens
10090
Mus musculus
tags
resistance markers :
1925
1487
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA