This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSLQ1658-dCas9-EGFP
catalog :
51023
citations: 16
Reference
Englert D, Burger E, Gr xfc n F, Verma M, Lackner J, Lampe M, et al. Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging. Nat Commun. 2023;14:3879 pubmed publisher
Hou Y, Wang D, Lu S, Guo D, Li M, Cui M, et al. Optogenetic Control of Background Fluorescence Reduction for CRISPR-Based Genome Imaging. Anal Chem. 2022;94:8724-8731 pubmed publisher
Ouyang J, Yadav T, Zhang J, Yang H, Rheinbay E, Guo H, et al. RNA transcripts stimulate homologous recombination by forming DR-loops. Nature. 2021;594:283-288 pubmed publisher
Lu S, Wang D, Hou Y, Guo D, Deng Y, Zhang X. Illuminating single genomic loci in live cells by reducing nuclear background fluorescence. Sci China Life Sci. 2020;: pubmed publisher
Chaudhary N, Nho S, Cho H, Gantumur N, Ra J, Myung K, et al. Background-suppressed live visualization of genomic loci with an improved CRISPR system based on a split fluorophore. Genome Res. 2020;30:1306-1316 pubmed publisher
Li P, Meng Y, Wang Y, Li J, Lam M, Wang L, et al. Nuclear localization of Desmoplakin and its involvement in telomere maintenance. Int J Biol Sci. 2019;15:2350-2362 pubmed publisher
Mao S, Ying Y, Wu X, Krueger C, Chen A. CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci. Nucleic Acids Res. 2019;47:e131 pubmed publisher
Guo D, Wang D, Liu C, Lu S, Hou Y, Zhang X. CRISPR-based genomic loci labeling revealed ordered spatial organization of chromatin in living diploid human cells. Biochim Biophys Acta Mol Cell Res. 2019;1866:118518 pubmed publisher
Smith E, Garza Gongora A, MacQuarrie K, Kosak S. Interstitial telomeric loops and implications of the interaction between TRF2 and lamin A/C. Differentiation. 2018;102:19-26 pubmed publisher
Wu X, Mao S, Yang Y, Rushdi M, Krueger C, Chen A. A CRISPR/molecular beacon hybrid system for live-cell genomic imaging. Nucleic Acids Res. 2018;46:e80 pubmed publisher
Stanyte R, Nuebler J, Blaukopf C, Hoefler R, Stocsits R, Peters J, et al. Dynamics of sister chromatid resolution during cell cycle progression. J Cell Biol. 2018;217:1985-2004 pubmed publisher
Schuijers J, Manteiga J, Weintraub A, Day D, Zamudio A, Hnisz D, et al. Transcriptional Dysregulation of MYC Reveals Common Enhancer-Docking Mechanism. Cell Rep. 2018;23:349-360 pubmed publisher
Neguembor M, Sebastian Perez R, Aulicino F, Gomez Garcia P, Cosma M, Lakadamyali M. (Po)STAC (Polycistronic SunTAg modified CRISPR) enables live-cell and fixed-cell super-resolution imaging of multiple genes. Nucleic Acids Res. 2018;46:e30 pubmed publisher
Choudhury S, Cui Y, Lubecka K, Stefanska B, Irudayaraj J. CRISPR-dCas9 mediated TET1 targeting for selective DNA demethylation at BRCA1 promoter. Oncotarget. 2016;7:46545-46556 pubmed publisher
Fu Y, Rocha P, Luo V, Raviram R, Deng Y, Mazzoni E, et al. CRISPR-dCas9 and sgRNA scaffolds enable dual-colour live imaging of satellite sequences and repeat-enriched individual loci. Nat Commun. 2016;7:11707 pubmed publisher
Chen B, Gilbert L, Cimini B, Schnitzbauer J, Zhang W, Li G, et al. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell. 2013;155:1479-91 pubmed publisher
product information
Catalog Number :
51023
Product Name :
pSLQ1658-dCas9-EGFP
article :
doi10.1016/j.cell.2013.12.001
id7755
pubmed_id24360272
bacterial resistance :
Ampicillin
cloning :
backbonepMSCVpuro
backbone_mutationNone
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
CRISPR
growth strain :
Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)
growth temp :
We suggest using Stellar (Clontech) for growth
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterMSCV LTR promoter
sequencing_primer_3ggctgctaaagcgcatgct
sequencing_primer_5ctcgatcctccctttatccagcc
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
namedCas9 fuse to EGFP
shRNA_sequence
size4887
species
9606
Homo sapiens
tags
locationC terminal on insert
tagEGFP
resistance markers :
1925
1487
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA