This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLKO.1-puro U6 sgRNA Oct4A -12
catalog :
50921
citations: 1
Reference
Kearns N, Genga R, Enuameh M, Garber M, Wolfe S, Maehr R. Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014;141:219-23 pubmed publisher
product information
Catalog Number :
50921
Product Name :
pLKO.1-puro U6 sgRNA Oct4A -12
article :
doi10.1242/dev.103341
id8011
pubmed_id24346702
bacterial resistance :
Ampicillin
cloning :
backbonepLKO.1
backbone_mutation
backbone_origin
backbone_size7145
promoterU6
sequencing_primer_3CCTCGAGCCGCGGCCAAAG
sequencing_primer_5CGTGACGTAGAAAGTAATAATTTC
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth notes :
gRNA target sequence GTGGGACTGGGGAGGGAGAG
growth strain :
U6 driven sgRNA targeting OCT4 isoform A -12 bp from TSS
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BfuAi
cloning_site_5BfuAI
promoterU6
sequencing_primer_3CCTCGAGCCGCGGCCAAAG
sequencing_primer_5CGTGACGTAGAAAGTAATAATTTC
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesOCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
genePOU5F1
id5460
genbank_ids
mutation
nameOct4A -12 sgRNA
shRNA_sequence
size7132
species
9606
Homo sapiens
tags
resistance markers :
1900
584
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA