This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHAGE EF1 dCas9-VP64
catalog :
50918
citations: 20
Reference
Marks B, Pipia I, Mukai C, Horibata S, Rice E, Danko C, et al. GDNF-RET signaling and EGR1 form a positive feedback loop that promotes tamoxifen resistance via cyclin D1. BMC Cancer. 2023;23:138 pubmed publisher
Sarno F, Goubert D, Logie E, Rutten M, Koncz M, Deben C, et al. Functional Validation of the Putative Oncogenic Activity of PLAU. Biomedicines. 2022;11: pubmed publisher
Oo J, P xe1 lfi K, Warwick T, Wittig I, Prieto Garcia C, Matkovic V, et al. Long non-coding RNA PCAT19 safeguards DNA in quiescent endothelial cells by preventing uncontrolled phosphorylation of RPA2. Cell Rep. 2022;41:111670 pubmed publisher
Nair V, Mayer J, Vincendeau M. A protocol for CRISPR-mediated activation and repression of human endogenous retroviruses in human pluripotent stem cells. STAR Protoc. 2022;3:101281 pubmed publisher
Chang L, Li M, Shao S, Li C, Ai S, Xue B, et al. Nuclear peripheral chromatin-lamin B1 interaction is required for global integrity of chromatin architecture and dynamics in human cells. Protein Cell. 2022;13:258-280 pubmed publisher
Yue J, Dai Q, Hao S, Zhu S, Liu X, Tang Z, et al. Suppression of the NTS-CPS1 regulatory axis by AFF1 in lung adenocarcinoma cells. J Biol Chem. 2021;:100319 pubmed publisher
Dibitetto D, Sims J, Ascenção C, Feng K, Kim D, Oberly S, et al. Intrinsic ATR signaling shapes DNA end resection and suppresses toxic DNA-PKcs signaling. NAR Cancer. 2020;2:zcaa006 pubmed publisher
Stojic L, Lun A, Mascalchi P, Ernst C, Redmond A, Mangei J, et al. A high-content RNAi screen reveals multiple roles for long noncoding RNAs in cell division. Nat Commun. 2020;11:1851 pubmed publisher
Rankin C, Shao L, Elliott J, Rowe L, Patel A, Videlock E, et al. The IBD-associated long noncoding RNA IFNG-AS1 regulates the balance between inflammatory and anti-inflammatory cytokine production after T-cell stimulation. Am J Physiol Gastrointest Liver Physiol. 2020;318:G34-G40 pubmed publisher
Hsu M, Liao H, Truong V, Huang K, Yu F, Chen H, et al. CRISPR-based Activation of Endogenous Neurotrophic Genes in Adipose Stem Cell Sheets to Stimulate Peripheral Nerve Regeneration. Theranostics. 2019;9:6099-6111 pubmed publisher
Perry R, Hezroni H, Goldrich M, Ulitsky I. Regulation of Neuroregeneration by Long Noncoding RNAs. Mol Cell. 2018;72:553-567.e5 pubmed publisher
Goubert D, Koncz M, Kiss A, Rots M. Establishment of Cell Lines Stably Expressing dCas9-Fusions to Address Kinetics of Epigenetic Editing. Methods Mol Biol. 2018;1767:395-415 pubmed publisher
Bischoff F, Werner A, John D, Boeckel J, Melissari M, Grote P, et al. Identification and Functional Characterization of Hypoxia-Induced Endoplasmic Reticulum Stress Regulating lncRNA (HypERlnc) in Pericytes. Circ Res. 2017;121:368-375 pubmed publisher
Good C, Madzo J, Patel B, Maegawa S, Engel N, Jelinek J, et al. A novel isoform of TET1 that lacks a CXXC domain is overexpressed in cancer. Nucleic Acids Res. 2017;45:8269-8281 pubmed publisher
Song J, Cano Rodriquez D, Winkle M, Gjaltema R, Goubert D, Jurkowski T, et al. Targeted epigenetic editing of SPDEF reduces mucus production in lung epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2017;312:L334-L347 pubmed publisher
Cui J, Rothstein M, Bennett T, Zhang P, Xia N, Reijo Pera R. Quantification of dopaminergic neuron differentiation and neurotoxicity via a genetic reporter. Sci Rep. 2016;6:25181 pubmed publisher
Saayman S, Lazar D, Scott T, Hart J, Takahashi M, Burnett J, et al. Potent and Targeted Activation of Latent HIV-1 Using the CRISPR/dCas9 Activator Complex. Mol Ther. 2016;24:488-98 pubmed publisher
Himeda C, Jones T, Jones P. CRISPR/dCas9-mediated Transcriptional Inhibition Ameliorates the Epigenetic Dysregulation at D4Z4 and Represses DUX4-fl in FSH Muscular Dystrophy. Mol Ther. 2016;24:527-35 pubmed publisher
Pham H, Kearns N, Maehr R. Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian Cells. Methods Mol Biol. 2016;1358:43-57 pubmed publisher
Kearns N, Genga R, Enuameh M, Garber M, Wolfe S, Maehr R. Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014;141:219-23 pubmed publisher
product information
Catalog Number :
50918
Product Name :
pHAGE EF1 dCas9-VP64
article :
doi10.1242/dev.103341
id8011
pubmed_id24346702
bacterial resistance :
Ampicillin
cloning :
backbonepHAGE
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth notes :
Please note that there are several mismatches (minor deletions and insertions) between depositor's reference sequence and Addgene's quality control sequence. The mismatches are in cloning junctions/non-coding regions and should not affect plasmid function.
growth strain :
Constitutive dCas9-VP64 lentiviral expression vector
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterEF1alpha
sequencing_primer_3MSCV-rev
sequencing_primer_5AGAGCTCGTTTAGTGAACCG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationD10A, H840A
namedCas9
shRNA_sequence
size4406
species
32644
Other
S. pyogenes
tags
locationC terminal on insert
tag3xHA
locationC terminal on insert
tagVP64
resistance markers :
1900
584
tags :
High Copy
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA