This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHAGE TRE dCas9-KRAB
catalog :
50917
citations: 26
Reference
Fetiva M, Liss F, Gertzmann D, Thomas J, Gantert B, Vogl M, et al. Oncogenic YAP mediates changes in chromatin accessibility and activity that drive cell cycle gene expression and cell migration. Nucleic Acids Res. 2023;51:4266-4283 pubmed publisher
Beucher A, Miguel Escalada I, Balboa D, De Vas M, Maestro M, García Hurtado J, et al. The HASTER lncRNA promoter is a cis-acting transcriptional stabilizer of HNF1A. Nat Cell Biol. 2022;24:1528-1540 pubmed publisher
Voloudakis G, Vicari J, Venkatesh S, Hoffman G, Dobrindt K, Zhang W, et al. A translational genomics approach identifies IL10RB as the top candidate gene target for COVID-19 susceptibility. NPJ Genom Med. 2022;7:52 pubmed publisher
Groen K, van Nieuwkoop S, Lamers M, Fouchier R, van den Hoogen B. Evidence against the Human Metapneumovirus G, SH, and M2-2 Proteins as Bona Fide Interferon Antagonists. J Virol. 2022;96:e0072322 pubmed publisher
Huo D, Yu Z, Li R, Gong M, Sidoli S, Lu X, et al. CpG island reconfiguration for the establishment and synchronization of polycomb functions upon exit from naive pluripotency. Mol Cell. 2022;82:1169-1185.e7 pubmed publisher
Li J, Ohmura S, Marchetto A, Orth M, Imle R, Dallmayer M, et al. Therapeutic targeting of the PLK1-PRC1-axis triggers cell death in genomically silent childhood cancer. Nat Commun. 2021;12:5356 pubmed publisher
Mazor G, Smirnov D, Ben David H, Khrameeva E, Toiber D, Rotblat B. TP73-AS1 is induced by YY1 during TMZ treatment and highly expressed in the aging brain. Aging (Albany NY). 2021;13:14843-14861 pubmed publisher
Xiong F, Wang R, Lee J, Li S, Chen S, Liao Z, et al. RNA m6A modification orchestrates a LINE-1-host interaction that facilitates retrotransposition and contributes to long gene vulnerability. Cell Res. 2021;31:861-885 pubmed publisher
Li R, Xia X, Wang X, Sun X, Dai Z, Huo D, et al. Generation and validation of versatile inducible CRISPRi embryonic stem cell and mouse model. PLoS Biol. 2020;18:e3000749 pubmed publisher
Chen L, Shi K, Qiu W, Aagaard L, Kassem M. Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation. Stem Cells Int. 2020;2020:8857344 pubmed publisher
Wei L, Wang L, Pan J, Liu L, Wang P, Wei J, et al. Specificity and application of SOX2 antibody. Poult Sci. 2020;99:2385-2394 pubmed publisher
Creed M, Baldeosingh R, Eberly C, Schlee C, Kim M, Cutler J, et al. PAX-SIX-EYA-DACH Network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis. Development. 2019;: pubmed publisher
Neiman G, Scarafía M, La Greca A, Santín Velazque N, Garate X, Waisman A, et al. Integrin alpha-5 subunit is critical for the early stages of human pluripotent stem cell cardiac differentiation. Sci Rep. 2019;9:18077 pubmed publisher
Morton A, Dogan Artun N, Faber Z, Macleod G, Bartels C, Piazza M, et al. Functional Enhancers Shape Extrachromosomal Oncogene Amplifications. Cell. 2019;179:1330-1341.e13 pubmed publisher
Musa J, Cidre Aranaz F, Aynaud M, Orth M, Knott M, Mirabeau O, et al. Cooperation of cancer drivers with regulatory germline variants shapes clinical outcomes. Nat Commun. 2019;10:4128 pubmed publisher
Gruber J, Chen J, Geller B, Jäger N, Lipchik A, Wang G, et al. Chromatin Remodeling in Response to BRCA2-Crisis. Cell Rep. 2019;28:2182-2193.e6 pubmed publisher
Weston S, Matthews K, Lent R, Vlk A, Haupt R, Kingsbury T, et al. A yeast suppressor screen used to identify mammalian SIRT1 as a proviral factor for MERS-CoV replication. J Virol. 2019;: pubmed publisher
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de Boer H, Pool M, Joosten E, Everts M, Samplonius D, Helfrich W, et al. Quantitative proteomics analysis identifies MUC1 as an effect sensor of EGFR inhibition. Oncogene. 2019;38:1477-1488 pubmed publisher
Tan Y, Jin C, Ma W, Hu Y, Tanasa B, Oh S, et al. Dismissal of RNA Polymerase II Underlies a Large Ligand-Induced Enhancer Decommissioning Program. Mol Cell. 2018;71:526-539.e8 pubmed publisher
Khan M, Xiang S, Song Z, Wu M. The p53-inducible long noncoding RNA TRINGS protects cancer cells from necrosis under glucose starvation. EMBO J. 2017;36:3483-3500 pubmed publisher
Ho S, Hartley B, Flaherty E, Rajarajan P, Abdelaal R, Obiorah I, et al. Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes. Stem Cell Reports. 2017;9:615-628 pubmed publisher
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Genga R, Kearns N, Maehr R. Controlling transcription in human pluripotent stem cells using CRISPR-effectors. Methods. 2016;101:36-42 pubmed publisher
Pham H, Kearns N, Maehr R. Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian Cells. Methods Mol Biol. 2016;1358:43-57 pubmed publisher
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product information
Catalog Number :
50917
Product Name :
pHAGE TRE dCas9-KRAB
article :
doi10.1242/dev.103341
id8011
pubmed_id24346702
bacterial resistance :
Ampicillin
cloning :
backbonepHAGE
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
CRISPR
growth notes :
Please note that there are several mismatches (minor deletions and insertions) between depositor's reference sequence and Addgene's quality control sequence. The mismatches are in cloning junctions/non-coding regions and should not affect plasmid function.
growth strain :
Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterTRE
sequencing_primer_3
sequencing_primer_5AGAGCTCGTTTAGTGAACCG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationD10A, H840A (catalytically inactive)
namedCas9
shRNA_sequence
size4460
species
32644
Other
S. pyogenes
tags
locationC terminal on insert
tag3xHA
locationC terminal on insert
tagKRAB
resistance markers :
1900
584
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA