pHAGE TRE dCas9-VP64 | Addgene 50916 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pHAGE TRE dCas9-VP64

catalog :

50916

citations: 9

Reference
Marks B, Pipia I, Mukai C, Horibata S, Rice E, Danko C, et al. GDNF-RET signaling and EGR1 form a positive feedback loop that promotes tamoxifen resistance via cyclin D1. BMC Cancer. 2023;23:138 pubmed publisher
Thaler R, Khani F, Sturmlechner I, Dehghani S, Denbeigh J, Zhou X, et al. Vitamin C epigenetically controls osteogenesis and bone mineralization. Nat Commun. 2022;13:5883 pubmed publisher
Saratov V, Ngo Q, Pedot G, Sidorov S, Wachtel M, Niggli F, et al. CRISPR activation screen identifies TGFβ-associated PEG10 as a crucial tumor suppressor in Ewing sarcoma. Sci Rep. 2022;12:10671 pubmed publisher
Chen L, Shi K, Qiu W, Aagaard L, Kassem M. Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation. Stem Cells Int. 2020;2020:8857344 pubmed publisher
de Boer H, Pool M, Joosten E, Everts M, Samplonius D, Helfrich W, et al. Quantitative proteomics analysis identifies MUC1 as an effect sensor of EGFR inhibition. Oncogene. 2019;38:1477-1488 pubmed publisher
Sun S, Xiao J, Huo J, Geng Z, Ma K, Sun X, et al. Targeting ectodysplasin promotor by CRISPR/dCas9-effector effectively induces the reprogramming of human bone marrow-derived mesenchymal stem cells into sweat gland-like cells. Stem Cell Res Ther. 2018;9:8 pubmed publisher
Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, et al. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust reactivation of the HIV-1 latent reservoirs. Sci Rep. 2015;5:16277 pubmed publisher
Pham H, Kearns N, Maehr R. Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian Cells. Methods Mol Biol. 2016;1358:43-57 pubmed publisher
Kearns N, Genga R, Enuameh M, Garber M, Wolfe S, Maehr R. Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014;141:219-23 pubmed publisher

product information

Catalog Number :

50916

Product Name :

pHAGE TRE dCas9-VP64

article :

doi	10.1242/dev.103341
id	8011
pubmed_id	24346702

bacterial resistance :

Ampicillin

cloning :

backbone

pHAGE

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

CRISPR

growth notes :

Please note that there are several mismatches (minor deletions and insertions) between depositor's reference sequence and Addgene's quality control sequence. The mismatches are in cloning junctions/non-coding regions and should not affect plasmid function.

growth strain :

Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector

origin :

pi :

alt_names

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	TRE
sequencing_primer_3
sequencing_primer_5	AGAGCTCGTTTAGTGAACCG
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

D10A, H840A nuclease-deficient

name

dCas9

shRNA_sequence

size

4406

species

32644

Other

S. pyogenes