pHAGE TRE dCas9 | Addgene 50915 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pHAGE TRE dCas9

catalog :

50915

citations: 4

Reference
Wei L, Wang L, Pan J, Liu L, Wang P, Wei J, et al. Specificity and application of SOX2 antibody. Poult Sci. 2020;99:2385-2394 pubmed publisher
Beltran M, Tavares M, Justin N, Khandelwal G, Ambrose J, Foster B, et al. G-tract RNA removes Polycomb repressive complex 2 from genes. Nat Struct Mol Biol. 2019;26:899-909 pubmed publisher
Pham H, Kearns N, Maehr R. Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian Cells. Methods Mol Biol. 2016;1358:43-57 pubmed publisher
Kearns N, Genga R, Enuameh M, Garber M, Wolfe S, Maehr R. Cas9 effector-mediated regulation of transcription and differentiation in human pluripotent stem cells. Development. 2014;141:219-23 pubmed publisher

product information

Catalog Number :

50915

Product Name :

pHAGE TRE dCas9

article :

doi	10.1242/dev.103341
id	8011
pubmed_id	24346702

bacterial resistance :

Ampicillin

cloning :

backbone

pHAGE

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

CRISPR

growth notes :

Please note that there are several mismatches (minor deletions and insertions) between depositor's reference sequence and Addgene's quality control sequence. The mismatches are in cloning junctions/non-coding regions and should not affect plasmid function.

growth strain :

Tet-regulatable dCas9 lentiviral expression vector

origin :

pi :

alt_names

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	TRE
sequencing_primer_3
sequencing_primer_5	AGAGCTCGTTTAGTGAACCG
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

D10A, H840A

name

dCas9

shRNA_sequence

size

4312

species

32644

Other

S. pyogenes