This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-N1 human cofilin S3E
catalog :
50861
citations: 4
Reference
Cazzaro S, Fang C, Khan H, Witas R, Kee T, Woo J, et al. Slingshot homolog-1 mediates the secretion of small extracellular vesicles containing misfolded proteins by regulating autophagy cargo receptors and actin dynamics. Front Aging Neurosci. 2022;14:933979 pubmed publisher
Swiatlowska P, Sit B, Feng Z, Marhuenda E, Xanthis I, Zingaro S, et al. Pressure and stiffness sensing together regulate vascular smooth muscle cell phenotype switching. Sci Adv. 2022;8:eabm3471 pubmed publisher
Zalli D, Neff L, Nagano K, Shin N, Witke W, Gori F, et al. The Actin-Binding Protein Cofilin and Its Interaction With Cortactin Are Required for Podosome Patterning in Osteoclasts and Bone Resorption In Vivo and In Vitro. J Bone Miner Res. 2016;31:1701-12 pubmed publisher
Garvalov B, FLYNN K, Neukirchen D, Meyn L, Teusch N, Wu X, et al. Cdc42 regulates cofilin during the establishment of neuronal polarity. J Neurosci. 2007;27:13117-29 pubmed
product information
Catalog Number :
50861
Product Name :
pEGFP-N1 human cofilin S3E
article :
doi10.1523/JNEUROSCI.3322-07.2007
id7718
pubmed_id18045906
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_originInvitrogen
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
expresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cells
origin :
37
pi :
alt_names
CFL1
cloning
clone_methodRestriction Enzyme
cloning_site_3XmaI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3EGFP-N
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCFL, HEL-S-15, cofilin
geneCFL1
id1072
genbank_ids
mutationS3E psuedo-phosporylated, non-activatable
namecofilin 1 S3E
shRNA_sequence
size500
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagEGFP
resistance markers :
1811
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA