This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGFP-N1 human cofilin WT
catalog :
50859
citations: 14
Reference
Romero L, Caires R, Kaitlyn Victor A, Ramirez J, Sierra Valdez F, Walsh P, et al. Linoleic acid improves PIEZO2 dysfunction in a mouse model of Angelman Syndrome. Nat Commun. 2023;14:1167 pubmed publisher
Cazzaro S, Fang C, Khan H, Witas R, Kee T, Woo J, et al. Slingshot homolog-1 mediates the secretion of small extracellular vesicles containing misfolded proteins by regulating autophagy cargo receptors and actin dynamics. Front Aging Neurosci. 2022;14:933979 pubmed publisher
Hylton R, Heebner J, Grillo M, Swulius M. Cofilactin filaments regulate filopodial structure and dynamics in neuronal growth cones. Nat Commun. 2022;13:2439 pubmed publisher
Swiatlowska P, Sit B, Feng Z, Marhuenda E, Xanthis I, Zingaro S, et al. Pressure and stiffness sensing together regulate vascular smooth muscle cell phenotype switching. Sci Adv. 2022;8:eabm3471 pubmed publisher
Larbret F, Biber P, Dubois N, Ivanov S, Lafanechere L, Tartare Deckert S, et al. Deubiquitinase Inhibitors Impair Leukemic Cell Migration Through Cofilin Oxidation and Alteration of Actin Reorganization. Front Pharmacol. 2021;12:778216 pubmed publisher
Ullo M, Logue J. ADF and cofilin-1 collaborate to promote cortical actin flow and the leader bleb-based migration of confined cells. elife. 2021;10: pubmed publisher
Muscarella A, Dai W, Mitchell P, Zhang W, Wang H, Jia L, et al. Unique cellular protrusions mediate breast cancer cell migration by tethering to osteogenic cells. NPJ Breast Cancer. 2020;6:42 pubmed publisher
Di Cio S, Iskratsch T, Connelly J, Gautrot J. Contractile myosin rings and cofilin-mediated actin disassembly orchestrate ECM nanotopography sensing. Biomaterials. 2020;232:119683 pubmed publisher
Wille C, Eiseler T, Langenberger S, Richter J, Mizuno K, Radermacher P, et al. PKD regulates actin polymerization, neutrophil deformability, and transendothelial migration in response to fMLP and trauma. J Leukoc Biol. 2018;104:615-630 pubmed publisher
Zalli D, Neff L, Nagano K, Shin N, Witke W, Gori F, et al. The Actin-Binding Protein Cofilin and Its Interaction With Cortactin Are Required for Podosome Patterning in Osteoclasts and Bone Resorption In Vivo and In Vitro. J Bone Miner Res. 2016;31:1701-12 pubmed publisher
Kumar R, Janjanam J, Singh N, Rao G. A new role for cofilin in retinal neovascularization. J Cell Sci. 2016;129:1234-49 pubmed publisher
Sengelaub C, Navrazhina K, Ross J, Halberg N, Tavazoie S. PTPRN2 and PLCβ1 promote metastatic breast cancer cell migration through PI(4,5)P2-dependent actin remodeling. EMBO J. 2016;35:62-76 pubmed publisher
Aeberhard L, Banhart S, Fischer M, Jehmlich N, Rose L, Koch S, et al. The Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components. PLoS Pathog. 2015;11:e1004883 pubmed publisher
Garvalov B, FLYNN K, Neukirchen D, Meyn L, Teusch N, Wu X, et al. Cdc42 regulates cofilin during the establishment of neuronal polarity. J Neurosci. 2007;27:13117-29 pubmed
product information
Catalog Number :
50859
Product Name :
pEGFP-N1 human cofilin WT
article :
doi10.1523/JNEUROSCI.3322-07.2007
id7718
pubmed_id18045906
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_originInvitrogen
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Forward primer sequence: CGGAATTCACCACCATGGCCTCCGGTGTGGCTGTCTC. Reverse primer sequence: TTAACCCGGGATAACAAAGGCTTGCCCTCCAGGG
growth strain :
expresses human cofilin fused to EGFP in mammalian cells
origin :
37
pi :
alt_names
CFL1
cloning
clone_methodRestriction Enzyme
cloning_site_3XmaI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3EGFP-N
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCFL, HEL-S-15, cofilin
geneCFL1
id1072
genbank_ids
mutation
namecofilin 1
shRNA_sequence
size500
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagEGFP
resistance markers :
1811
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA