pmRFP-N1 human cofilin S3E | Addgene 50858 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pmRFP-N1 human cofilin S3E

catalog :

50858

citations: 5

Reference
Meka D, Scharrenberg R, Zhao B, Kobler O, König T, Schaefer I, et al. Radial somatic F-actin organization affects growth cone dynamics during early neuronal development. EMBO Rep. 2019;20:e47743 pubmed publisher
Sousa Squiavinato A, Rocha M, Barcellos de Souza P, de Souza W, Morgado Diaz J. Cofilin-1 signaling mediates epithelial-mesenchymal transition by promoting actin cytoskeleton reorganization and cell-cell adhesion regulation in colorectal cancer cells. Biochim Biophys Acta Mol Cell Res. 2019;1866:418-429 pubmed publisher
Bagnato P, Castagnino A, Cortese K, Bono M, Grasso S, Bellese G, et al. Cooperative but distinct early co-signaling events originate from ERBB2 and ERBB1 receptors upon trastuzumab treatment in breast cancer cells. Oncotarget. 2017;8:60109-60122 pubmed publisher
Tilve S, Difato F, Chieregatti E. Cofilin 1 activation prevents the defects in axon elongation and guidance induced by extracellular alpha-synuclein. Sci Rep. 2015;5:16524 pubmed publisher
Garvalov B, FLYNN K, Neukirchen D, Meyn L, Teusch N, Wu X, et al. Cdc42 regulates cofilin during the establishment of neuronal polarity. J Neurosci. 2007;27:13117-29 pubmed

product information

Catalog Number :

50858

Product Name :

pmRFP-N1 human cofilin S3E

article :

doi	10.1523/JNEUROSCI.3322-07.2007
id	7718
pubmed_id	18045906

bacterial resistance :

Kanamycin

cloning :

backbone

pmRFP-N1

backbone_mutation

backbone_origin

R.Y. Tsien, PNAS 2002 v99 pp7877 7882

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Please note that Addgene's sequencing results identified a F56L mutation in the mRFP sequence. According to the depositing laboratory, this does not affect the RFP fluorescence.

growth strain :

expresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cells

origin :

pi :

alt_names

CFL1

cloning

clone_method	Restriction Enzyme
cloning_site_3	XmaI
cloning_site_5	EcoRI
promoter	CMV
sequencing_primer_3	mRFP1-R
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CFL, HEL-S-15, cofilin
gene	CFL1
id	1072

genbank_ids

mutation

S3E psuedo-phosporylated, non-activatable

name

cofilin 1 S3E

shRNA_sequence

size

500

species

9606

Homo sapiens