This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1 human cofilin S3A
catalog :
50854
citations: 3
Reference
Ullo M, Logue J. ADF and cofilin-1 collaborate to promote cortical actin flow and the leader bleb-based migration of confined cells. elife. 2021;10: pubmed publisher
Zalli D, Neff L, Nagano K, Shin N, Witke W, Gori F, et al. The Actin-Binding Protein Cofilin and Its Interaction With Cortactin Are Required for Podosome Patterning in Osteoclasts and Bone Resorption In Vivo and In Vitro. J Bone Miner Res. 2016;31:1701-12 pubmed publisher
Garvalov B, FLYNN K, Neukirchen D, Meyn L, Teusch N, Wu X, et al. Cdc42 regulates cofilin during the establishment of neuronal polarity. J Neurosci. 2007;27:13117-29 pubmed
product information
Catalog Number :
50854
Product Name :
pcDNA3.1 human cofilin S3A
article :
doi10.1523/JNEUROSCI.3322-07.2007
id7718
pubmed_id18045906
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1(-)
backbone_mutation
backbone_originInvitrogen
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Human cofilin 1 cDNA was PCRed with a 5' primer that had an EcoRI, while the 3' primer had KpnI and EcoRV. EcoRI and KpnI were then used to ligate into pcDNA3.1- (leaving the EcoRV on the 3 end of the insert).
growth strain :
expresses constituvely active cofilin in mammalian cells
origin :
37
pi :
alt_names
CFL1
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3BGHrev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCFL, HEL-S-15, cofilin
geneCFL1
id1072
genbank_ids
mutationconstitutively active, S3A
namecofilin 1 S3A
shRNA_sequence
size500
species
9606
Homo sapiens
tags
resistance markers :
1811
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA