pcDNA3.1 human cofilin WT | Addgene 50853 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pcDNA3.1 human cofilin WT

catalog :

50853

citations: 2

Reference
Reggi E, Kaiser S, Sahnane N, Uccella S, La Rosa S, Diviani D. AKAP2-anchored protein phosphatase 1 controls prostatic neuroendocrine carcinoma cell migration and invasion. Biochim Biophys Acta Mol Basis Dis. 2024;1870:166916 pubmed publisher
Garvalov B, FLYNN K, Neukirchen D, Meyn L, Teusch N, Wu X, et al. Cdc42 regulates cofilin during the establishment of neuronal polarity. J Neurosci. 2007;27:13117-29 pubmed

product information

Catalog Number :

50853

Product Name :

pcDNA3.1 human cofilin WT

article :

doi	10.1523/JNEUROSCI.3322-07.2007
id	7718
pubmed_id	18045906

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1(-)

backbone_mutation

backbone_origin

Invitrogen

backbone_size

5428

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Human cofilin 1 cDNA was PCRed with a 5' primer that had an EcoRI, while the 3' primer had KpnI and EcoRV. EcoRI and KpnI were then used to ligate into pcDNA3.1- (leaving the EcoRV on the 3 end of the insert). Forward primer sequence: CGGAATTCACCACCATGGCCTCCGGTGTGGCTGTCTC. Reverse primer sequence: GGGGTACCGATATCCTCACAAAGGCTTGCCCTCCAGG.

growth strain :

expresses human cofilin in mammalian cells

origin :

pi :

alt_names

CFL1

cloning

clone_method	Restriction Enzyme
cloning_site_3	KpnI
cloning_site_5	EcoRI
promoter	CMV
sequencing_primer_3	BGHrev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CFL, HEL-S-15, cofilin
gene	CFL1
id	1072

genbank_ids

mutation

name

cofilin 1

shRNA_sequence

size

500

species

9606

Homo sapiens